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Volume 9, Issue 5 (Suppl)
J Bioequiv Availab, an open access journal
ISSN: 0975-0851
Pharmacy & Biopharma 2017
August 31-September 01, 2017 Philadelphia, USA
August 31-September 01, 2017 Philadelphia, USA
3
rd
International Conference on
Biopharmaceutics and Biologic Drugs
&
5
th
International Pharmacy Conference
J Bioequiv Availab 2017, 9:5 (Suppl)
DOI: 10.4172/0975-0851-C1-031
Curcumin potentiates the function of human α7-nicotinic acetylcholine receptors expressed in SH-EP1 cells
Murat Oz
1
, Eslam El Nebrisi
2
, Keun-Hang Susan Yang
3
and
Nadine Kabbani
4
1
Qatar University, Qatar
2
UAE University, UAE
3
Chapman University, USA
4
George Mason University, USA
E
ffects of curcumin, a biologically active ingredient of turmeric, was tested on the Ca2+ transients induced by the activation
of α7 subunit of the human nicotinic acetylcholine (α7 nACh) receptor expressed in SH-EP1 cells. Curcumin caused a
significant potentiation of choline (1 mM)-induced Ca2+ transients with an EC50 value of 231 nM. The potentiating effect
of curcumin was not observed in Ca2+ transients induced by high K+ (60 mM) containing solutions or activation of α4β2
nACh receptors. Notably, the effect of curcumin was not observed when curcumin and choline were co-applied without
curcumin pre-incubation. The effect of curcumin on choline-induced Ca2+ transients was not reversed by pre-incubation
with inhibitors of protein C, A, and CaM kinases. Metabolites of curcumin such as tetrahydrocurcumin, demethylcurcumin,
and didemethylcurcumin also caused potentiation of choline-induced Ca2+ transients. Collectively, our results indicate that
curcumin directly potentiate the function of the α7-nACh receptor expressed in SH-EP1 cells.
murat.oz@qu.edu.qa