pharmacy-biopharma-2017-posters-accepted-abstracts

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Volume 9, Issue 5 (Suppl)

J Bioequiv Availab, an open access journal

ISSN: 0975-0851

Pharmacy & Biopharma 2017

August 31-September 01, 2017 Philadelphia, USA

August 31-September 01, 2017 Philadelphia, USA

International Conference on

Biopharmaceutics and Biologic Drugs

International Pharmacy Conference

J Bioequiv Availab 2017, 9:5 (Suppl)

DOI: 10.4172/0975-0851-C1-031

Development and cytotoxicity evaluation of respirable nanomicelle carriers for delivery of tretinoin by

jet nebulizer

Maryam Rezaeizadeh, Abbas Pardakhty

and

Hamid Forutanfar

Kerman University of Medical Sciences, Iran

Background & Objectives:

Lung cancers are serious and lethal problems in cigarette smoking patients. Direct deposition of

cytotoxic drugs to the site of neoplasm (lungs) and avoiding the systemic side effects and drug interactions are some benefits

following inhalation of anticancer agents which can be an effective and safe alternative to systemic administration. The aim of

present study is to prepare and characterize chitosan-stearic acid conjugate nanomicelles for encapsulation of all-trans retinoic

acid (ATRA).

Methods:

Water soluble chitosan was grafted to stearic acid (SA) chains via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide

mediated coupling reaction. The chemical structure of depolymerized chitosan (DC)-SA copolymers and degree of amino

substitution was determined by 1H NMR. ATRA loaded micelles were prepared by film hydration, solvent evaporation and

dialysis method. The physicochemical properties and formation of polymeric micelles were studied by dynamic light scattering

and fluorescence spectroscopy methods. Nanomicelle size and zeta potential and ATRA entrapment efficiency were determined

and the cytotoxicity of the formulations was also evaluated on A549 cell line by MTT assay. ATRA-loaded micelles were also

characterized for their nebulization efficiency and retention of ATRA in the micelles after nebulization.

Results:

ATRA was loaded in nanomicelles with entrapment efficiencies more than 70%. Nanomicelles possessed positive

charges with mean particle sizes of less than 300 nm. The IC50 of ATRA nanomicelles showed increased cytotoxic potential

of drug. Transmission electron microscopy (TEM) revealed the spherical shape of prepared nanomicelles. The nebulization

efficiency was up to 89% and the fine particle fraction (FPF) varied from 38% to 47%. The micelles had enough stability to

remain encapsulation of the drug during nebulization process.

Conclusions:

The results exhibited the potential of DC-SA micelles as a suitable carrier for delivery of ATRA by different

routes of administration, specially the pulmonary route via jet nebulization.