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Volume 8
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
Pharma Biotech 2018
December 10-11, 2018
December 10-11, 2018 | Rome, Italy
23
rd
International Conference on
Pharmaceutical Biotechnology
Sequential/parallel production of potential malaria vaccines – a direct way from single batch to quasi-
continuous integrated production
Reiner Luttmanna
1
and
Sven-Oliver Borcherta
2
1
Hamburg University of Applied Sciences, Germany
2
Biomedical Primate Research Center, Netherlands
A
n intensification of pharmaceutical protein production processes can be achieved by the integration of unit operations and
application of recurring sequences of all biochemical process steps. Within optimization procedures each individual step
as well as the overall process has to be in the focus of scientific interest. This paper includes a description of the development
of a fully automated production plant, starting with a two-step upstream followed by a four step downstream line, including
cell clarification, broth cleaning with microfiltration, product concentration with ultrafiltration and purification with column
chromatography. Recursive production strategies are developed where a cell breeding, the protein production and the whole
downstream is operated in series but also in parallel, each main operation shifted by one day. The quality and reproducibility of
the recursive protein expression is monitored on-line by golden batch and this is controlled by model predictive multivariate
control (MPMC). As a demonstration process the production of potential malaria vaccines with Pichia pastoris is under
investigation.
reiner.luttmann@haw-hamburg.deJ Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C8-110