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Volume 8
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
Pharma Biotech 2018
December 10-11, 2018
December 10-11, 2018 | Rome, Italy
23
rd
International Conference on
Pharmaceutical Biotechnology
Britannin induces apoptosis through targeting AMPK in human breast cancer
Marzieh Moeinifard
1
and
Faranak Fallahian
2
1
Tarbiat Modares University, Iran
2
Qom University of Medical Sciences, Iran
N
owadays, there is an urgent need for novel drugs with improved efficiency against tumor cells. Of note, induction of cell
death pathways with regard to molecular targets is considered as a promising treatment method in cancerous cells. AMP-
activated protein kinase (AMPK) which is involved in suppression of cell growth and induction of apoptosis is emerged as
an attractive target molecule for cancer treatment. Recently, naturally derived products such as britannin had drawn growing
attention as an agent in cancer therapy. Britannin was extracted from Inula aucheriana by using chromatography methods.
Human breast cancer cell line (MCF-7) and normal breast cancer cell line (MCF10A) were applied to investigation of anticancer
properties of britannin. Cytotoxic effects of britannin were examined by MTT assay. Apoptosis induction was evaluated by
flow cytometry and also, activity of caspase3 was assessed by colorimetric assay. Furthermore, intracellular changes in protein
expression of Bax, Bcl2 and AMPK were analyzed by western blotting method. The viability of MCF-7 and MCF10A cells
inhibited after 24h treatment by britannin with the IC50 values 15±2.3µM and 80±5.4µM, respectively. Apoptosis induction
in MCF-7 cells was confirmed by annexin V-FITC/PI staining and caspase3 activation. In addition, britannin decreased
the expression of Bcl2 (anti-apoptotic protein) and increased the expression of Bax (pro-apoptotic protein) in MCF-7 cells.
Moreover, britannin increased phosphorylated active form of AMPK in MCF-7 cells. Taken together, the data shows that the
cytotoxic effect of britannin on the cancerous cells is significantly higher than on normal cells. Furthermore, results demonstrate
that britannin induces mitochondrial-apoptosis through activation of AMPK in breast cancer cells and may potentially serve
as an adjuvant agent for the treatment of human breast cancer.
Marzieh.moeinifard@modares.ac.irJ Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C8-110