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Volume 8
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
Pharma Biotech 2018
December 10-11, 2018
December 10-11, 2018 | Rome, Italy
23
rd
International Conference on
Pharmaceutical Biotechnology
Use of 3'-untranslated region of messenger RNA from measles virus matrix protein as a stabilizing
cis-element for increased protein production yields
Marzieh Marzbany
1
, Fatemeh Ghassemi
2
and
Mahsa Rasekhian
1
1
Kermanshah University of Medical Sciences, Iran
2
Pasteur Institute of Iran, Iran
T
he market for the use of recombinant proteins for medical applications has been increasing in recent years. Expression
vectors are one of the decisive factors in the cost-effectiveness of the production process of recombinant proteins. The
genetic factors found at the 3’untranslated region of mRNA expressed by such vectors, play an important role in determining
its stability and thus in the efficiency of the recombinant protein production process. We studied the effect of 3'UTR of matrix
protein mRNA from measles virus on mRNA stability by a GFP-based reporter construct in three cell lines. Application of
3'UTR of matrix protein mRNA from measles virus increased the GFP-mRNA stability in a time and cell dependent manner.
Analysis of the 3'UTR of matrix protein mRNA from measles virus for presence of known cis-acting motifs indicated the
occurrence of two PABPC1 binding sites, known for its stability and translation enhancing effects. Our results verified the
potentiality of 3'UTR region of matrix protein mRNA for improvement of recombinant protein production and vector design
for mammalian cell hosts.
marzehmarzbany@yahoo.comJ Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C8-110