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Euro Biotechnology 2016
November 07-09, 2016
Volume 6, Issue 7(Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
conferenceseries .com
November 07-09, 2016 Alicante, Spain
12th Euro Biotechnology Congress
Laura Katherine Rodriguez Sanchez et al., J Biotechnol Biomater 2016, 6:7(Suppl)
Callus induction and secondary metabolites production in Piper cf. cumanense Kunth (Piperaceae)
Laura Katherine Rodriguez Sanchez1, Xavier Marquinez1, Jorge E Perez1Luis E Cuca1, Juliet A Prieto2 and Oscar J Patino1
1Universidad Nacional de Colombia, Colombia
2Pontificia Universidad Javeriana, Colombia
Piper cf. cumanense is a species of which have been isolated some compounds with promissory bioactivity, particularly to
pest control in economically important crops and with potential use in parasitic diseases. However, these compounds have
been obtained in low concentrations being important to use biotechnology strategies in order to obtain more quantities of
bioactive substances. This study contributes to the investigations on this species in the development of strategies that improve
the production of these compounds. Mature seeds were placed in MS medium supplemented with 3% (w/v) sucrose and 0.02
mg/L of gibberellic acid (AG3) for germination. Laminas and petioles were excised from young plants obtained in vitro and
were cultured in MS medium supplemented with benzylaminopurine (BAP) and 2,4-dichlorophenoxyacetic acid (2,4-D) in
combination at different concentrations for organogenesis and callogenesis establishment. Excised young plants and callus were
freeze-dried, powdered and extracted with ethyl acetate and analyzed by HPLC. The major production of friable callus was
obtained using petiole as explant on MS medium supplemented with 2,4-D (1.0 mg/L) and BAP (0.5 mg/L). Callus presented
oxidation and to overcome it, some strategies were used being frequent subcultures the most effective. HPLC chromatograms
exhibited different patterns in major metabolites of the organs evaluated highlighting differences between in vitro and ex vitro
leaves. This research provides basis for applying different strategies to increase metabolites production on in vitro cultures and
for the development of biosynthetic studies.
Biography
Laura Katherine Rodriguez Sanchez is a Biologist, currently pursuing MSc in Biotechnology as a Member of the research group ‘Estudio químico y de actividad
biológica de Rutaceae y Myristicaceae colombianas’ of the Universidad Nacional de Colombia on the lines of plant biotechnology, phytochemistry and
chemoprospecting. Her areas of interest on research are plant biotechnology, phytochemistry and plant physiology.