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Euro Biotechnology 2016
November 07-09, 2016
Volume 6, Issue 7(Suppl)
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
conferenceseries
.com
November 07-09, 2016 Alicante, Spain
12
th
Euro Biotechnology Congress
Nyadar Palmah et al., J Biotechnol Biomater 2016, 6:7(Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.064Virus before oligonucleotide-vent to apoptosis (VOVA) effect as a promising tool for more effective
use of baculoviral preparations
Nyadar Palmah
1
, Oberemok V
1
, Laikova K
1
, Zaitsev A
1
, Adeyemi T
1
, Shumskykh M
1
, Temirova Z
1
, Kot F
1
, Kot T
1
, Meschryakova E
1
, Gerasimenko D
1
, Gal'chinsky
N
1
, Kenyol I M
1
, Reznik N G
1
and Skorokhod O A
2
1
V I Vernadsky Crimean Federal University, Crimea
2
University of Torino, Italy
I
n our studies concerning the elaboration of DNA insecticides, we found that topically applied antisense oligoRING
(5'-CGACGTGGTGGCACGGCG-3') from conservative region of IAP-3 (inhibitor of apoptosis) gene of
Lymantria dispar
multicapsid nuclear polyhedrosis virus (LdMNPV) causes significantly higher mortality of
Lymantria dispar
caterpillars and
decrease of host
IAP-1
gene expression in LdMNPV-infected insect cells. Also using DNA ladder assay for apoptosis detection
we found that oligoRING triggers stronger apoptotic processes in infected insect cells in comparison with water-treated
control and groups with control oligonucleotides. Demonstrated insecticidal effect of the oligoRING on LdMNPV-infected
Lymantria dispar
has also been observed in our investigations with LdMNPV-infected
Lymantria monacha
and LdMNPV-
infected
Cydalima perspectalis
. We decided to term this phenomenon described for 3 lepidopteran pests as VOVA (Virus before
Oligonucleotide-Vent to Apoptosis) effect. Of note, we could not reach VOVA effect with LdMNPV and oligoRING on dipteran
Drosophila melanogaster
and coleopteran
Leptinotarsa decemlineata
what shows specificity of VOVA effect/many lepidopteran
pests in horticulture and forestry are successfully controlled by application of baculoviruses. Baculoviral preparations have
proven to be selective and expensive, acting effectively but slowly. The slow action of a baculovirus is associated with a latent
period in the life cycle of the virus. Discovered insecticidal effect of the viral oligoRING on LdMNPV-infected
L. dispar, L.
monacha, C. perspectalis
is a fundamental finding for a host-virus system that could be applied in biologically based insect pest
management together with baculoviral preparations to provide more effective and faster action of the latter.
Biography
Nyadar Palmah is currently a Postgraduate student under the supervision of Oberemok Volodymyr at the V.I. Vernadsky University, Simferopol, Crimea.
biopalmgene@gmail.com