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Euro Biotechnology 2016

November 07-09, 2016

Volume 6, Issue 7(Suppl)

J Biotechnol Biomater

ISSN: 2155-952X JBTBM, an open access journal

conferenceseries

.com

November 07-09, 2016 Alicante, Spain

12

th

Euro Biotechnology Congress

Akli Ouelhadj, J Biotechnol Biomater 2016, 6:7(Suppl)

http://dx.doi.org/10.4172/2155-952X.C1.064

Isolation and screening of actinomycetes from Algerian soil for their enzymatic and antimicrobial

activities

Akli Ouelhadj

Mouloud Mammeri University, Algeria

T

he constant evolution of bacterial resistance to antibiotics and the emergence of new infectious diseases is a major public

health problem; hence the urgent need for new antimicrobial molecules. Actinomycetes, slow growing Gram-positive

bacteria are known as an organism that is useful in the search for bioactive compounds. In this study, 27 isolates of actinomycetes

were isolated from soil samples collected in the area of Tizi Ouzou region (northern Algeria). A significant difference in the

number of colonies was observed between the different culture media (Bennett, GLM and Sabouraud). After purification,

the antibacterial activity of the 27 actinomycetes strains was tested against three bacterial strains from the ATCC collection

(

Escherichia coli

ATCC 25922,

Pseudomonas aeruginosa

ATCC 27853 and

Staphylococcus aureus

ATCC 25923) by two agar

diffusion methods: the perpendicular streak method and the agar cylinder method. Among 27 isolated strains, 13 showed

antibacterial activity toward at least one bacterium in the primary screening. Of the 13 strains showing an antibacterial activity,

5 of them were found to be highly active against

Staphylococcus aureus

with inhibition diameters ranging from 20 to 26 mm.

The 27 isolates were then subjected for enzymatic activities. From the test, only 3 strains of isolates have the ability to degrade

cellulose; 4 showed amylase and 3 protease activities. The isolates showed positive results were then selected for identification.

Biography

Akli Ouelhadj has completed his PhD at Martin Luther University, Germany and Postdoctoral studies from Pen State University, USA. Since 2009, he is an

Assistant Professor at the Department of Biochemistry and Microbiology, University of Mouloud Mammeri, Algeria. His main interests focuses on microbial and plant

biotechnology, genetics, bioactives compounds and abiotic stress.

ouelhadj_akli@hotmail.com