euro-biotechnology-2016_scientifictracks-abstracts

Notes:

Page 36

Euro Biotechnology 2016

November 07-09, 2016

Volume 6, Issue 7(Suppl)

J Biotechnol Biomater

ISSN: 2155-952X JBTBM, an open access journal

conferenceseries

.com

November 07-09, 2016 Alicante, Spain

Euro Biotechnology Congress

V K Gunathilake et al., J Biotechnol Biomater 2016, 6:7(Suppl)

Immunosuppressive and anti cancer activity of a novel Sri Lanka marine sponge,

Haliclona (Soestella)

species

V K Gunathilake, E D de Silva and P V Udagama

University of Colombo, Sri Lanka

arine sponge extracts are increasingly utilized as immunosuppressive and anticancer therapeutic agents. This study

aimed to investigate the immunosuppressive activity and cytotoxicity of presumably a novel Sri Lankan sponge species,

Haliclona (Soestella)

sp., the crude sponge extract (HSCE) and its purified fractions were tested for these activities by the

MTT dye reduction assay on Wistar rat bone marrow cells (BMCs) and on the Hep-2 human larynx carcinoma cell line,

respectively. Sponge samples were harvested from Unawatuna, Galle, Sri Lanka by scuba diving and refluxed thoroughly with

methanol/dichlorommethane, followed by filtration and rota evaporation. The resultant HSCE was subjected to solvent-solvent

partitioning with chloroform, ethyl acetate, hexane and water. The HSCE and its fractions (10, 100, 1000, 2000 and 5000 µg/

mL) were tested ex vivo on BMC proliferation by the MTT assay. Cytotoxicity of the HSCE and its chloroform fraction (CF)

were tested at 6.25, 12.5, 25, 50 and 100 µg/mL on the Hep-2 cell line. BMC proliferation evidenced dose dependant percentage

inhibition by the HSCE with significant proliferation inhibition observed in 1000 and 2000 µg/mL concentrations (P<0.05; IC

0.719 µg/mL for 2000 µg/mL dose). Highest (100%) inhibition was exhibited by the CF followed by the ethyl acetate fraction

(60-70% inhibition) with no inhibition by water and hexane fractions. Cytotoxicity of HSCE and CF on Hep-2 cells reported

values of 19.7 and 29.7 µg per mL, respectively. In conclusion, the HSCE and its fractions were immunosuppressive with

respect to BMC proliferation, while HSCE and CF showed cytotoxicity against human larynx carcinoma cells.