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Volume 6, Issue 4 (Suppl)
Clin Pharmacol Biopharm, an open access journal
ISSN: 2167-065X
Page 85
Euro Biopharma & Ethnopharmacology 2017
November 09-11, 2017
&
6
th
International Conference and Exhibition on
November 09-11, 2017 Vienna, Austria
4
th
EUROPEAN BIOPHARMA CONGRESS
PHARMACOLOGY AND ETHNOPHARMACOLOGY
Joint Event
Evaluation of antiangiogenic and antitumor properties of anogeissus leiocarpus
Loiy Elsir Ahmed Hassan1,
2
*, Aman Shah Abdul Majid
3
, Foud S Al-Suade
1
, Saad S Dahham
1
and
Amin Malik Shah Abdul Majid
1
1
EMAN Research and Testing Laboratory, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Malaysia
2
Omdurman Islamic University, Department of Pharmacology, Malaysia
Background:
Anogeissus leiocarpus is a traditional medicinal plant with strong antioxidant and hypoglycemic properties.
Hypothesis/Purpose:
This study was aimed to investigate the antiangiogenic and cytotoxic effects of eight extracts from
Anogeissus leiocarpa (leaves and bark).
Study Design:
Eventually, the most active extract was subjected to a series of
in vitro
and
in vivo
studies to elucidate the
mechanism of action.
Methods:
In order to confirm the effect of the extract onmotility of human endothelial cells, cell migration assay was conducted.
In addition, VEGF suppressive effect of the extract was assessed in endothelial cells. Finally, the antitumor effect of the extract
was evaluated using
in vivo
human tumor xenograft model.
Results:
Results of the present study indicated that, hexane extract of the stem bark of A. leiocarpus was found as the most
active extract on inhibition of sprouting of microvessels (89.56%). Additionally, ethanol extract of the leaves exerted high
antiangiogenic (inhibition 82.12%) in rat aortic ring assay. Hexane extract of the stem bark displayed significant inhibitory
effect on endothelial cells proliferation (76.87 %) while ethanol extract of the leaves was save on HUVEC cell lines (inhibition
5.80%). The two extracts inhibited HUVEC migration by 87.57 and 65.23% respectively. The extracts demonstrated significant
inhibition of VEGF levels (45.32 and 30.52 % respectively) in treated endothelial cells. Finally the extracts exhibited potent
anti-tumorigneic effect in anthymic mice with ∆T/∆C =8.43 and 12.54% at doses 400 and 200mg/kg, respectively.
Conclusion:
These results may provide novel guidelines towards improved strategies using Anogeissus leiocarpus extracts
based on the suppression of angiogenesis to curb the growth of tumors. The plant can be used as promising candidate for anti-
neoplastic drug development.
Key words:
Medicinal plants, Antitumor
in vivo
, antiangiogenic Anogeissus leiocarpus
loiy.ahmed23@gmail.comClin Pharmacol Biopharm 2017, 6:4(Suppl)
DOI: 10.4172/2167-065X-C1-026