35 / 41
Volume 6, Issue 4 (Suppl)
Clin Pharmacol Biopharm, an open access journal
ISSN: 2167-065X
Page 89
Euro Biopharma & Ethnopharmacology 2017
November 09-11, 2017
&
6
th
International Conference and Exhibition on
November 09-11, 2017 Vienna, Austria
4
th
EUROPEAN BIOPHARMA CONGRESS
PHARMACOLOGY AND ETHNOPHARMACOLOGY
Joint Event
Phytochemical screening and antioxidant activity of cardiospermum corindum l faux persil from
botswana
Motlhanka DMT*, Montsho S
and
Nthoiwa, K K
Botswana University of Agriculture and Natural Resources, Botswana
T
he aim of this study was to compare the phytochemical composition and antioxidant activity of roots and shoots of
Cardiospermum corindum collected from two geographically distant regions of Botswana (Tswapong Hills and Kgale
Hills). Qualitative phytochemical analysis revealed presence of alkaloids, reducing sugars, saponins, phytosterols, phenols,
flavonoids and terpenoids. Analysis by thin layer chromatography, revealed that both shoots and roots of plant collected from
the two respective regions showed no differences in phytochemical constituents. Total phenol and flavonoid contents were
quantitatively estimated. Total phenolic content measured by Folin-Ciocalteu method varied from 164.4±2.2 to 364.2±3.1mg/L
(GAE) Gallic Acid Equivalents. The order of total phenol contents were [364.2±3.1](Roots from Tswapong Hills)>[356.0±4.5]
(Roots from Kgale Hills)>[169.1±2.6](Shoots from Tswapong Hills)>[164.4±2.2mg/lGAE](Shoots from Kgale Hills). The
total flavonoid contents as measured by aluminium Chloride method varied from 56.7±1.1 to 124.1±1.5mg/L(QE) Quercetin
Equivalents. The order of the total flavonoid contents were [124.1±1.5](Shoots from Kgale Hills)>[118.8±2.6](Shoots from
Tswapong Hills)>[63.3±1.6](Roots from Tswapong Hills)>[56.7±1.1mg/l QE](Roots from Kgale Hills). The antioxidant
activity as determined by the DPPH radical scavenging assay, revealed that, at all tested concentrations, root extracts exhibited
greater (≥86%) scavenging potency than shoot extracts (≤83%). A direct correlation between total phenolic content and free
radical scavenging activity was revealed. This work has validated the use of this plant as a health improving tool. However,
structural identification of the bioactive constituents should be carried out.
motlhankadan@yahoo.comClin Pharmacol Biopharm 2017, 6:4(Suppl)
DOI: 10.4172/2167-065X-C1-026