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Volume 6, Issue 4 (Suppl)
Clin Pharmacol Biopharm, an open access journal
ISSN: 2167-065X
Page 83
Euro Biopharma & Ethnopharmacology 2017
November 09-11, 2017
&
6
th
International Conference and Exhibition on
November 09-11, 2017 Vienna, Austria
4
th
EUROPEAN BIOPHARMA CONGRESS
PHARMACOLOGY AND ETHNOPHARMACOLOGY
Joint Event
Antioxidant activity and hepatoprotective effects of
Centaurea incana
on CCL
4
-induced liver toxicity
in rats
Boubellouta Houria
1
, Khelifi Touhami Fatima
2
, Kermandji Mohamed Azed
2
and
Bellatrache Cherifa
2
1
University of Bejaia, Algeria
2
University of Constantine 1, Algeria
Aim:
The aim of the present study was to investigate the potential antioxidant and hepatoprotective effects of
Centaurea incana
on the free radical damage of liver caused by carbon tetrachloride in rats.
Methods:
For the study of preventive effect of methanolic extract of
Centaurea incana
on CCl
4
–induced hepatotoxicity, our
study was carried out on rats. The animals were randomly divided in to 4 different groups comprising 7 animals each. Group
I served as controls and received an injection of vehicle (olive oil) alone; Acute liver injury in rats was induced by a single
intraperitoneal injection with CCl
4
dissolved in an equal volume of olive oil at a dose of 3 ml/kg body weight, group II, which
is well documented to induce hepatotoxicity. Group III was administered methanolic extract of
Centaurea incana
at a dose
of 500 mg/kg alone. Group IV was administered methanolic extract of
Centaurea incana
at a dose of 500 mg/kg and was
injected by CCl
4
i.p., at a dose of 3 ml/kg body weight. After 4 weeks of treatment, all of the animals were sacrificed 24 h after
administration of CCl4, and blood was collected, serum was separated and stored at −20°C.
Results:
The single intraperitoneal injection with CCl
4
caused severe hepatotoxicity in rats, as evidenced by the significant
elevation of serum AST and ALT activities after the administration of CCl4. The concentration of MDA, an end product of
lipid peroxidation, in the rats treated with CCl
4
was increased 2.7-fold when compared with the vehicle control rats. However,
pre-treatment with
Centaurea incana
significantly prevented the elevation of serum AST and ALT activities induced by CCl
4
treatment. Consistent with the serum AST and ALT activities, pre-treatment with
Centaurea incana
for 4 weeks to the rats
resulted in a significant decrease in the concentration of hepatic MDA when compared with the CCl
4
group.
Conclusion:
Our investigation provided convincing data that
Centaurea incana
decrease the lipid per-oxidation and liver
enzymes, and increase the anti-oxidant defense system activity in the CCl4-treated rats. The mechanisms underlying
hepatoprotection of the methanolic extract of
Centaurea incana
may be related to both its radical scavenging properties and
indicate effects as a regulator of antioxidative systems.
khelifi_t_fatima@yahoo.frClin Pharmacol Biopharm 2017, 6:4(Suppl)
DOI: 10.4172/2167-065X-C1-026