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Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
MLC as an effective tool in the prediction of human intestinal absorption
D S Shokry, L J Waters and G M B Parkes
University of Huddersfield, UK
Estimation of human intestinal absorption is very important especially for orally administered pharmaceutical compounds
with poor solubility. Therefore in pre-formulation studies the extent of drug absorption must be determined for new drug
entities (NDE). The use of animals has been the most abundant method used in pre-formulation studies for determination
of pharmacokinetics, especially, the rate and extent of intestinal absorption of pharmaceutical compounds. In this work the
use of biodetergent based micellization with a form of chromatography known as micellar liquid chromatography (MLC)
has been successful in the determination of human intestinal absorption. Bile salts were used as a mobile phase in the MLC
chromatographic method to provide an environment more closely simulating the human intestinal environment. In this
method intestinal absorption was successfully predicted by the use of a group of model compounds through measurement
and calculation of the partition coefficient, Pmw. A series of model drugs were prepared in the corresponding mobile phase
concentration and injected into the chromatographic system with capacity factors obtained by analysis of the retention data
recorded for the model drugs used. Experimentally determined partition coefficients were used alongside other molecular
descriptors in the modeling of human intestinal absorption (% HIA) using multiple linear regression. The obtained model
confirmed the ability of MLC to predict human intestinal absorption (% HIA) (R2=0.86 and R2Pred.=0.75).
Biography
D S Shokry has achieved her first degree in Pharmacy in 2009 from Ain Shams University and has completed her Master’s degree in Pharmaceutical Analytical
Chemistry from Cairo University in 2013. She is currently working towards her PhD as a Member of Dr. L Waters group for finding alternatives to animal testing
at Huddersfield University. She has worked as a Teaching Assistant and as an Assistant Lecturer in the Analytical Chemistry department at the Future University,
Egypt. She has published three papers in reputed journals and presented her work as oral/poster presentations at five conferences.
Shokry@dinashokri
D S Shokry et al., J Chromatogr Sep Tech 2016, 7:5(Suppl)