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Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
Performance evaluation of different columns in hydrophilic interaction liquid chromatography for
the determination of N-nitrosodiethanolamine in shampoo
G Abedi and Z Talebpour
Alzahra University, Iran
N-nitrosodiethanolamine (NDELA) as a non-volatile and carcinogen nitrosamine has been detected in a wide variety of
cleaning products, cosmetic raw materials and products. Several methods have been developed to detect and determine
NDELA. In this study, a novel hydrophilic interaction liquid chromatography (HILIC) method has been introduced for
determination of NDELA as a polar compound in shampoos. For this purpose, different columns were utilized and their
global resolutions and asymmetry factors were acquired in order to evaluate chromatographic performance of each column.
Separation was achieved on CN, Si, NH2, and Zwitterionic (ZIC) columns. The flow rate and injection volume were 0.8 mL
min-1 and 20 μL, respectively, with UV detection at 234 nm wavelength. In order to optimize mobile phase (MP) composition,
content of acetate buffer varied from 0 to 10%. The obtained results were shown retention of NDELA was increased with
decrease of water content of the MP, but at least 2% of water is needed for a sufficient hydration of the stationary phase particles.
Also pH and the concentration of the buffer were changed in the range of 3.7 to 7.7 and 5 to 100 mM, respectively. Based on
our results, the best separation conditions were chosen: acetonitrile/40 mM ammonium acetate, pH 4.7 (98:2, v/v) as the MP
and column temperature of 35ºC. With respect to the global resolution and asymmetry factor, ZIC column showed the best
performance followed by an NH2 column. The proposed method can be satisfactorily applied to the inspection of shampoos.
Biography
G Abedi is a PhD candidate in Analytical Chemistry at Alzahra University, Tehran, Iran. She has published 3 papers in ISI journals. Her current research interests
are Chromatographic Methods, Mass Spectrometry, Cosmetics and Detergent Industry, Pharmaceutical Industry.
G Abedi et al., J Chromatogr Sep Tech 2016, 7:5(Suppl)