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Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
Performance Comparison Between Monolithic ColumnAnd Silica Based C-18 Particle Packed Column
For The Determination Of Three Anti-diabetics In Pharmaceuticals
A.Hemdan1 , Omar Abdel-Aziz2
1Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Ahram Canadian University
2Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Ain Shams University
The performance of monolithic column was compared with C-18 particle packed column for the analysis of anti-diabetic
combination. Three drugs, Metformin, Glimepiride, and Pioglitazone was quantified by both monolithic and reversed
phase C-18column. The mobile phase used for monolithic column was consisting of potassium dihydrogen phosphate buffer
adjusted to pH 3 by o-phosphoric acid / acetonitrile (55/45 v/v) and the run time of the method was 6 minutes. While for
C-18 particle packed column the mobile phase was consisting of potassium dihydrogen phosphate buffer adjusted to pH 3.5
by o-phosphoric acid / acetonitrile (40/60 v/v) and the run time of the method was 9 minutes. The flow rate was 1.5 mL/min
for both methods. The HPLC methods using both columns were utilized for determination of the anti-diabetic drugs in bulk
powder and marketed pharmaceutical formulation. Monolithic column showed superior results in terms of resolution, run
time, peak symmetry, time and solvent saving, and finally back pressure stability.
Biography
Dr. A. Hemdan has completed his Ph.D at the age of 31 years from Ain Shams University. He is working as an Associate professor at Faculty of Pharmacy-Ahram
Canadian University. He has published more than 10 papers in reputed journals.
A.Hemdan et al., J Chromatogr Sep Tech 2016, 7:5(Suppl)