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Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
Analysis of endogenous aldehydes in human urine by static headspace gas chromatography–mass spectrometry
Manuel Silva and Maria Serrano
University of Córdoba, Spain
Endogenous aldehydes (EAs) are intermediary or final products of the metabolism involved in a wide spectrum of
biochemical and physiological processes, such as oxidative stress and cell processes. This research reports a solvent-free and
automated analytical method for the determination of EAs in human urine using a static headspace generator sampler coupled
with gas chromatography–mass spectrometry (HS–GC–MS). Twelve significant EAs used as markers of different biochemical
and physiological processes, namely short- and medium-chain alkanals, α,β-unsaturated aldehydes and dicarbonyl aldehydes
were selected as target analytes. Direct human urine samples (no dilution is required) were derivatized with O-2,3,4,5,6-
pentafluorobenzyl hydroxylamine in alkaline medium (hydrogen carbonate–carbonate buffer, pH 10.3). The analytical method
allows the simultaneous derivatization and extraction of EAs in human urine, completing the entire process in 20 min. The
HS–GC–MS method developed renders an efficient tool for the fast, sensitive and precise determination of EAs in human
urine with limits of detection from 1 to 15ng/L and relative standard deviations, (RSDs) from 6.0 to 7.9%. Average recoveries
by enriching urine samples ranged between 92 and 95%. Aldehydes were readily determined at 0.005–50 µg/L levels in human
urine from healthy subjects, smokers and diabetic adults. The twelve aldehydes under study were detected in the whole array
of human urine samples analyzed. The quantification of aldehydes in those samples showed significantly differences in their
concentrations when comparing smokers and diabetics to healthy subjects.
Biography
Manuel Silva completed his PhD in 1978 at University of Seville. He is Full Professor and Head of the Department of Analytical Chemistry at University of Córdoba.
He has published about 150 papers in reputed journals. In the last decade, his research has been focused on the detection of aldehydes as water disinfection
by-products and their distribution in treated water. He has directed 15 PhD theses and has worked in several organizations at the Ministry of Education and
Science.
Manuel Silva et al., J Chromatogr Sep Tech 2016, 7:5(Suppl)