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Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
Novel approach for targeted profiling of plant ecdysteroids by UHPLC-MS/MS vs. SFC-UV/MS
Danuse Tarkowska and Miroslav Strnad
Palacky University, Czech Republic
Plant ecdysteroids (PEs) are a family of about 300 polyhydroxylated triterpenoids related in structure to the major
invertebrate steroid hormone 20-hydroxyecdysone (20E). ECs were originally found in animal sources and recognized as
steroidal hormones controlling moulting (ecdysis) and metamorphosis in insects. Later, some of them were discovered to be
present also in terrestrial plant families. Their role in plants is still not fully elucidated but at least it is clear that they don’t have
hormonal function like in insects and serve the most probably as the defense against insect herbivores. The pharmacological
and medicinal studies in humans show that PEs act as very effective adaptogens and elicitors of anabolic effects on skeletal
muscle in a non-androgenic manner. Methods based on the use of HPLC have historically been most frequently used for
separating ecdysteroids isolated from different biological sources either in analytical or in preparative scale. However, in the
last decade, UHPLC and SFC experienced a great boom in separation science area. Here, we present new approach for analysis
of PEs that is based on UHPLC separation in combination with tandem MS detection. The selected analytes of PEs character
(20E, polypodine B, ajugasterone C, stachysterone C and ponasterone A) are separated within 4.5 min using RP column
and detected in MRM mode. The method provides sufficient chromatographic resolution and sensitivity with LOD ranging
between 0.12 and 5.4pg. To compare the results obtained by UHPLC-(+ESI)-MS/MS technique, we developed also the method
based on SFC with both UV and MS detection.
Biography
Danuse Tarkowska has completed her PhD from Palacky University and Post-doctoral studies from Umeå Plant Science Centre. She is Senior Researcher at the
Laboratory of Growth Regulators, Centre of the Region Hana for Biotechnological and Agricultural Research. His current focus is on the development of methods
for extraction and purification of plant hormones, analytical methods for plant hormones and other signaling molecules. She has published more than 40 papers in
reputed journals (sometimes cited without self-citations 872, h-index: 12). She is the member of Czech Chemical Society and Phytochemical Society of Europe.
Dana Tarkowska@dankatar
Danuse Tarkowska et al., J Chromatogr Sep Tech 2016, 7:5(Suppl)