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Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
Temperature controlled liquid phase microextraction, in-situ derivatization for determination of
estrogens in water by gas chromatography-tandem mass spectrometry
Maw-Rong Lee, Chung-Yu Chen and Yi-Yu Chen
National Chung Hsing University, Taiwan
Estrogens, one group of endocrine disrupting chemicals (EDCs), can easily accumulate in the human body and interfere
with organism’s endocrine system at low concentration. The trace estrogens in environmental water system have attracted
scientists’ and human attention. Therefore, to develop a simple, rapid, and high sensitive analytical method for determination of
the trace estrogens in environmental water is important for environmental safety. In this study, a novel temperature-controlled
liquid phase microextraction (TC-LPME) by in-situ derivatization coupled to gas chromatography-tandemmass spectrometry
(GC-MS/MS) for analyzing trace estrogens including estrone, 17α-estradiol, 17α-ethinylestradiol, mestranol, diethylstilbestrol,
and steinheim in water matrix has been developed. An aliquot of 11.25 mL water sample with pH 7 and containing 5% sodium
chloride reacted with 3.75 mL dansyl chloride (10 μg/mL) as derivatization agent has been prepared. After derivatization for
15 minutes at 60ºC, the derivatives were extracted by liquid phase microextraction with octanol as extraction solvent and
the extractant was analyzed by GC-MS/MS. The linearity of the proposed method was 0.05 to 50 ng/mL; with a coefficient of
determination above 0.9940. The limits of detection (LODs) of target estrogens were between 0.3 and 1.1 ng/mL. The intra-day
and inter-day precisions were between 1.2 and 10.8% and 2.9 and 17.6%, respectively. The recovery of the proposed method
ranged 84.9 and 107.8%. The results demonstrated that the proposed TC-LPME-GC-MS/MS method is rapid, simple, high
sensitive and high selective method for the determination of trace estrogens in environmental water matrix.
Biography
Maw-Rong Lee has completed his MS from University of Florida. He is Dean of College of Science and Distinguished Professor of Department of Chemistry at the
National Chung Hsing University, Taiwan. He has published more than 80 papers in reputed journals.
Maw-Rong Lee et al., J Chromatogr Sep Tech 2016, 7:5(Suppl)