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Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
Sensitive determination of phthalates in edible oils enabled through elimination of phthalate background
from HPLC–MS/MS
Adam Vavrouš1,2, Václav Ševčík1,2, Karel Vrbík1 and Alena Moulisová 1
1National Institute of Public Health, Czech Republic
2Charles University in Prague, Czech Republic
Diesters of phthalic acid (phthalates) are high production volume chemicals known for their endocrine-disrupting
properties. Phthatlate control in various types ofmatrixmay be quite complicated by omnipresent phthalate contamination.
Chemicals, laboratory equipment or GC instrumentation typically introduce considerable levels of phthalates into the analytical
process. Unfortunately, the role of phthalate contamination in HPLC remains unclear. For this reason, we attempted to identify
and eliminate any possible blank difficulties encountered during analysis of 6 individual phthalates (DEP, DBP, DiBP, BBP,
DEHP, DnOP) and 2 phthalate isomeric mixtures (DiNP and DiDP) in edible oils by HPLC–MS/MS. Several sources of
phthalate contamination were identified, however, the mobile phase was the most serious. The key improvement was achieved
by equipping a contamination trap, a HPLC column, generating a retention delay of mobile phase phthalates. LOQs ranging
between 5.5 and 110 µg/kg reflect satisfactory blank management and good sensitivity of the employed instrumentation.
Biography
Adam Vavrouš has graduated in 2010 from University of Chemistry and Technology, Prague. Since 2011, he has been employed as an Analytical Chemist/
Researcher at National Institute of Public Health. In 2012, he started PhD study at Charles University in Prague.
Adam Vavrouš et al., J Chromatogr Sep Tech 2016, 7:5(Suppl)