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Chromatography 2016
September 21-23, 2016
Volume 7, Issue 5(Suppl) J Chromatogr Sep Tech 2016
ISSN: 2157-7064 JCGST, an open access journal
conferenceseries .com
September 21-23, 2016 Amsterdam, Netherlands
World Congress on Chromatography
Identification of components from Sinapis semina that act on the thoracic aorta by screening using cell
membrane chromatography combined with online-high performance liquid chromatography-mass spectrum
Fen Wei and Sicen Wang
Xi’an Jiaotong University, China
S inapis semina (JieZi in Chinese), which is the dried seed of Sinapis alba L. (Brassicaceae), has been reported for having
antihypertensive efficacy. However, the active components have not been investigated. Since many antihypertensive drugs
act on receptors in the vasculature, we have developed a Sprague-Dawley (SD) rat thoracic aorta cell membrane chromatography
(CMC) coupled with HPLC/MS method, based on ligand-receptor interactions, to screen for active components in S. semina.
Firstly, a fractionwas recognized and retained by the CMC column.This retained fractionwas directed onto anODS enrichment
column, and then analyzed and identified by the HPLC/MS system through switching a two-position ten-port switch valve.
In this study, the activity and reproducibility of CMC column and the enrichment rate were investigated by nifedipine which
was used as a positive control. The results showed that our SD rat thoracic aortas CMC column was able to recognize receptor-
active compounds in a complex system. Both the reproducibility of enrichment and the enrichment rate met the experimental
requirements. Then, the methanol extract of S. semina was screened using this method. Sinapine, molecular weight 310 g/mol,
was identified as a potential antihypertensive compound. To confirm the effect of the active component from S. semina, tension
measurements were performed in vitro using isolated rat mesenteric arteries at a dose of 10-8-10-4 mol/L, with nifedipine as the
positive control. In vitro pharmacological experiments showed that sinapine was able to relax rat mesenteric artery rings. So,
sinapine may have a potential antihypertensive effect.
Biography
Fen Wei is a PhD candidate in School of Pharmacy at Xi’an Jiaotong University. She has participated in 11 published papers in different journals and has acquired
a patent as a participator. In addition, another three papers in which she is the first author are under review.
Fen Wei et al., J Chromatogr Sep Tech 2016, 7:5(Suppl)