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Volume 4 Toxicology: Open Access
ISSN: 2476-2067 Toxicology Congress 2018
March 12-14, 2018
March 12-14, 2018 Singapore
14th World Congress on
Toxicology and Pharmacology
Ionizing radiation induces developmental abnormalities but not lethality of sea urchin embryos
Mahmoud Ibrahim Shoulkamy1, Toshiaki Nakano2, Amir Salem3 and Hiroshi Ide2
1Minia University, Egypt
2Hiroshima University, Japan
3National Research Centre, Egypt
The Fukushima nuclear power plant accident occurred in Japan on March 2011, initiated by tsunami following a great
earthquake cause the discharged of radioactive materials into the environment. This accident received considerable
attention for their effect on marine ecosystems. Sea urchins are model organisms in developmental biology research and their
embryos are sensitive to toxins and used to study the developmental and cytological effects of anthropogenic pollutants and
environmental stressors. In the present study sea urchin embryos were used as a model system to assess the effect of ionizing
radiation on the viability and early development of marine invertebrate animals. Sea urchin embryos were culture in filtered
sea water at 16 °C at the different developmental stages were irradiated with X-rays (70 kV, 0.2 mm Al filter, dose rate=1.46
Gy/min) and further incubated in filtered sea water at 16 °C. Irradiation of embryos at the different stages of development (32-
cell, mid-gastrula and early Pluteus larva) at doses up to 30 Gy did not reduce the viability of embryos. However, irradiated
embryos exhibited dose-dependent developmental abnormalities. Typical abnormalities observed for gastrula embryos were
delayed development and a reduced number of primary and secondary mesenchyme cells and those for mid Pluteus larva were
delayed development, skeletal abnormalities, separated body rod tips and fused arms. Interestingly, the frequency of X-rays
induced abnormalities increases when embryos were irradiated before mid-blastula transition (MBT) and starts to decrease
thereafter. The analysis of apoptosis of X-ray irradiated embryos resulted in the absence of apoptotic response when embryos
were irradiated before MBT. However, there is immediate apoptotic response was observed when embryos were irradiated after
MBT.
Recent Publications
1. Shoulkamy M I, Nakano T, Ohshima M, Hirayama R, Uzawa A, Furusawa Y, Ide H (2012) Detection of DNA-protein
crosslinks (DPCs) by novel direct fluorescence labeling methods: distinct stabilities of aldehyde and radiation-induced
DPCs. Nucleic Acids Research; 40(18): e143.
2. Ide H, Shoulkamy M I, Nakano T, Miyamoto-Matsubara M, Salem A M (2011) Repair and biochemical effects of DNA-
protein crosslinks. Journal of Mutation Research; 711(1-2): 113-122.
Biography
Mahmoud Ibrahim Shoulkamy has his expertise in DNA damage response to environmental and anticancer agents and risk sciences of radiation and chemicals.
He has received his Doctoral degree from Graduate School of Science, Hiroshima University, Japan in 2013 in the field of DNA damage and its repair mechanisms.
In 2013, he was appointed as an Assistant Professor (special appointment) in Graduate School of Science, Hiroshima University, Japan until 2016 and currently he
is working as an Assistant Professor in Zoology Department, Faculty of Science, Minia University, Egypt.
Mahmoud Ibrahim Shoulkamy et al., Toxicol Open Access 2018, Volume 4
DOI: 10.4172/2476-2067-C1-005