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Volume 4 Toxicology: Open Access
ISSN: 2476-2067 Toxicology Congress 2018
March 12-14, 2018
March 12-14, 2018 Singapore
14th World Congress on
Toxicology and Pharmacology
A comprehensive method for the screening of drugs in severely decomposed human tissues by fast gas
chromatography/tandem mass spectroscopy (Fast GC-MS/MS)
Brian Waters, Kenji Hara, Natsuki Ikematsu, Mio Takayama, Aya Matsusue, Masayuki Kashiwagi and Shin-Ichi Kubo
Fukuoka University, Japan
Statement of the Problem: Drug screening is an important reference in forensic autopsy investigations. In postmortem
toxicology, often the samples provided for analysis are in a severe state of putrefaction or decomposition. The presence of
breakdown products such as lipids and amino acids make extraction of the compounds of interest difficult. Also, developing
an analytical method capable of detecting trace levels of analytes from the interfering substances present in these complex
matrices adds to the challenges.
Methodology & Theoretical Orientation: For this study, putrefied and decomposing tissue samples from actual cases
autopsied at our department were analyzed. Human tissue specimens consisted of liver, kidney, spleen, lung, muscle and brain,
if available. The drugs detected from these specimens included phenobarbital, chlorpromazine, promethazine, aripiprazole,
amlodipine, telmisartan, rosvastatine, chlorpheniramine, etizolam and zolpidem. Specimens of 0.3 g were treated with urease,
acidified or alkalized and extracted with acetonitrile. Lipid-removal and solid-phase extraction cartridges were employed
while carefully monitoring the pH of samples to ensure the adequate removal of interfering substances. The extracts were
evaporated and reconstituted in n-propyl acetate:methanol (1:1) for fast GC-MS/MS analysis.
Findings: The developed method was successful in clearly identifying drugs from putrefied specimens. The use of tandem
mass spectrometry helped to reduce the influence of background noise and interfering substances.
Conclusion & Significance: Putrefied specimens are often the only remaining samples left from severely decomposed cadavers.
The combination of a robust preparation method and analysis with fast GC-MS/MS could aid the forensic medicine and
toxicology communities in elucidating important information from these often-overlooked biological matrices.
Recent Publications
1. Hara K, Waters B, Ikematsu N, et al. (2016) Development of a preparation method to produce a single sample that can be
applied to both LC-MS/MS and GC-MS for the screening of postmortem specimens. Legal Medicine; 21: 85-92.
2. Waters B, Ikematsu N, Hara K, et al. (2016) GC-PCI-MS/MS and LC-ESI-MS/MS databases for the detection of 104
psychotropic compounds. Legal Medicine; 20: 1-7.
References
1. Butzbach D (2010) The influence of putrefaction and sample storage on post-mortem toxicology results. Forensic Science,
Medicine and Pathology; 6: 35-45.
2. Watterson J, Imfeld A, Cornthwaite H (2014) Determination of colchicine and O-demethylatedmetabolites in decomposed
skeletal tissues by microwave assisted extraction, microplate solid phase extraction and ultra-high performance liquid
chromatography (MAE-MPSPE-UHPLC). Journal of Chromatography B; 960: 145-150.
3. Wiebe T, Watterson J (2014) Analysis of tramadol and O-desmethyltramadol in decomposed skeletal tissues following acute
and repeated tramadol exposure by gas chromatography mass spectrometry. Forensic Science International; 242: 261-265.
Biography
Brian Waters has received his Master of Science degree in Criminalistics from California State University Los Angeles, USA. After working as a Criminalist for
the County of Los Angeles, Department of Coroner/Medical Examiner for almost 8 years, he has joined as an Assistant Professor in the Department of Forensic
Medicine at Fukuoka University in Japan. His specialty is postmortem forensic toxicology and he has published academic papers on fast gas chromatography-mass
spectrometry, the analysis of novel psychoactive compounds, preparation methods for postmortem samples and the analysis of volatile hydrocarbons in blood.
Brian Waters et al., Toxicol Open Access 2018, Volume 4
DOI: 10.4172/2476-2067-C1-005