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Volume 3, Issue 1 (Suppl) Toxicol Open Access
ISSN: 2476-2067 TYOA, an open access journal Toxicology Congress 2017
April 13-15, 2017
April 13-15, 2017 Dubai, UAE
8th World Congress on
Toxicology and Pharmacology
Polyphenol resveratrol triggers Cisplatin-resistant human oral cancer CAR cell autophagy and
apoptosis
Chi-Cheng Lu, Chao-Hsiang Chang and Jai-Sing Yang
China Medical University, Taiwan
Resveratrol is known to be an effective chemopreventive agent against multiple tumor cells, but the increasing drug
resistance avoids the cancer treatment in oral cavity cancer. In the current study, we investigated the oral anti-tumor
activity of resveratrol and its underlying mechanism in Cisplatin-resistant oral cancer CAR cells. Our results demonstrated
that resveratrol provoked autophagic cell death to form AVOs and autophagic vacuoles in CAR cells by acridine orange (AO)
and monodansylcadaverine (MDC) staining. Inhibitors of PI3K class III (3-MA) and AMP-activated protein kinase (AMPK)
(compound c) suppressed the autophagic vesicle formation, LC3-II protein levels and autophagy induced by resveratrol.
Z-VAD-FMK (pan-caspase inhibitor) attenuated resveratrol-triggered cleaved caspase-9, cleaved caspase-3 and cell apoptosis.
Resveratrol also enhanced phosphorylation of AMPK and regulated autophagy- and pro-apoptosis-related signals in
resveratrol-treated CAR cells. Our results indicated that resveratrol is likely to induce autophagic and apoptotic death in drug-
resistant oral cancer cells and might become a new approach for oral cancer treatment in the near future.
Biography
Chi-Cheng Lu completed his Graduation and Doctorate from National Chung Hsing University (NCHU), Taichung, Taiwan. He worked at the Department of Food
Science and Biotechnology of NCHU as a Post-doctoral Fellow under Chair Professor Gow-Chin Yen. He then worked with Research Fellow Dr Jai-Sing Yang of
China Medical University Hospital (Taiwan) as a Post-doctoral Research and Project Scientist. His specific interests are focused on targeting tumor cell demise
(apoptosis, autophagy and necrosis) and anti-metastatic effect in vitro and in vivo after exposure to phytochemicals and the synthesis of novel compounds. He has
also undertaken oxidative stress and functional food research in recent years and is exploring their molecular mechanisms.
Chi-Cheng Lu et al., Toxicol Open Access 2017, 3:1 (Suppl)