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Volume 3, Issue 1 (Suppl) Toxicol Open Access
ISSN: 2476-2067 TYOA, an open access journal Toxicology Congress 2017
April 13-15, 2017
April 13-15, 2017 Dubai, UAE
8th World Congress on
Toxicology and Pharmacology
Allyl isothiocyanate induces apoptotic mechanism via endoplasmic reticulum stress and mitochondrial
pathway in human colorectal cancer HT29 cells
Jo-Hua Chiang1 , Chi-Cheng Lu2 and Jai-Sing Yang2
1Chung-Jen Junior College of Nursing, Health Sciences and Management, Taiwan
2China Medical University, Taiwan
Allyl isothiocyanate (AITC), one of isothiocyanate (ITC) family found in a constituent of cruciferous vegetables, has
chemopreventive and antitumor activities in several cancers. However, no available information showed antitumor effects
on human colorectal cancer cells. The current study was focused to explore mechanisms underlying AITC-induced apoptosis
in human colorectal cancer HT29 cells. The results showed that AITC reduced cell number and viability using trypan blue stain
by countess automated cell counter as well as utilizing MTT assay, respectively. AITC also was observed to induce apoptotic cell
morphological changes by a contrast-phase microscope and cell cycle arrest at G2/M phase by flow cytometric assay in HT29
cells. Intrinsic apoptosis-associated factors such as caspase-9 and caspase-3 activities were performed. The levels of reactive
oxygen species (ROS) production, release of cytosolic Ca2+, loss of mitochondrial membrane potential (ΔΨm) occurred in
AITC-treated HT29 cells. AITC stimulated mitochondria-related signaling, including cytochrome c, Apaf-1, AIF and Endo G
in HT29 cells. We further found that calpain 1, ATF-6α, GRP78, GRP94, GADD153 and capase-4 signals were up-regulated
in HT29 cells after AITC challenge. Importantly, NAC (an ROS scavenger) and BAPTA (a selective Ca2+ chelator) abolished
AITC-reduced viability in HT29 cells. Additionally, AITC down-regulated CDK1 activity and altered the expressions of G2/M
phase-modulated associated protein levels by Western blotting in HT29 cells. Therefore, our findings demonstrated AITC can
promote G2/M phase arrest and trigger HT29 cell apoptosis through ER stress and mitochondria-dependent pathway. AITC
possibly exhibits as a potential anticancer agent and could be applied in the treatment of human colorectal cancer.
Biography
Jo-Hua Chiang received her PhD degree in Life Sciences at National Chung Hsing University (NCHU), Taichung, Taiwan. She then worked as a Post-doctoral
Fellow with distinguished chair Professor Tian-Shung Wu of the Department of Chemistry, National Cheng Kung University, Tainan, Taiwan. She is currently an
Assistant Professor at the Department of Nursing, Chung-Jen Junior College of Nursing, Health Sciences and Management, Chiayi County, Taiwan. She has
worked in the area of anticancer molecular signaling. Her fields of interest include phytochemicals, herbal medicine and the synthesis of novel compounds against
multiple cancer cells and antiangiogenic actions in vitro and in vivo.
Jo-Hua Chiang et al., Toxicol Open Access 2017, 3:1 (Suppl)