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Volume 3, Issue 1 (Suppl) Toxicol Open Access
ISSN: 2476-2067 TYOA, an open access journal Toxicology Congress 2017
April 13-15, 2017
April 13-15, 2017 Dubai, UAE
8th World Congress on
Toxicology and Pharmacology
Novel quinazoline compound-induced apoptotic cell death in 5FU-resistant HT29 cells
Je-Wei Tsao, Mann-Jen Hour and Jai-Sing Yang
China Medical University, Taiwan
In this study, Novel Quinazoline Compound (NQC) suppressed viability in 5FU-resistant HT29 cells through inhibiting cell
proliferation, causing cell cycle arrest and triggering apoptosis. Flow cytometry analysis revealed that NQC caused 5FU-
resistant HT29 cell cycle arrest at G2/M phase and increased the proportion of polyploidy cells. Western blotting indicated that
the expression of cyclin B1, p-Cdk1 and Cdk1 increased after treatment with NQC. Colorimetric assay analyses also showed
that activities of caspase-3, caspase-8 and caspase-9 occurred in NQC-treated 5FU-resistant HT29 cells. Together, these results
suggest that NQC inhibited 5FU-resistant HT29 cells growth through inducing mitotic catastrophe by interference with G2/M
cell cycle checkpoint which may open a new avenue for the treatment of 5FU-resistant colon cells.
Biography
Je-Wei Tsao is a Research Assistant of Department of Medical Research, China Medical University Hospital, China Medical University, Taiwan. His main research
interests are exploring molecular mechanisms of Herbal Medicine and new drugs against cancer development.
Je-Wei Tsao et al., Toxicol Open Access 2017, 3:1 (Suppl)