toxicology-congress-2017-posters-accepted-abstracts

10 / 28

Page 82
Notes:
conferenceseries.com
Volume 3, Issue 1 (Suppl) Toxicol Open Access
ISSN: 2476-2067 TYOA, an open access journal Toxicology Congress 2017
April 13-15, 2017
April 13-15, 2017 Dubai, UAE
8th World Congress on
Toxicology and Pharmacology
YC-1, soluble guanylate cyclase stimulator, induces G0/G1 arrest and apoptotic cell death in Cisplatin-
resistant human oral cancer CAR cells
Miau-Rong Lee, Meng-Wei Lin and Jai-Sing Yang
China Medical University, Taiwan
In this study, YC-1, a soluble guanylate cyclase stimulator, suppressed viability in Cisplatin-resistant human oral cancer CAR
cells via inhibiting cell proliferation, G0/G1 phase arrest and triggering apoptosis. Results from flow cytometry analysis
indicated that YC-1 promoted G0/G1 phase arrest and provoked apoptosis in CAR cells. YC-1 treatment up-regulated p21
and down-regulated cyclin A, D, E, CDK2 and protein expression. YC-1 caused apoptotic cell death and DNA fragmentation
evidenced by DAPI/TUNEL staining. YC-1 time-dependently disrupted the mitochondrial membrane potential (ΔΨm). It
enhanced the protein levels of cytochrome c, Bax, Bak as well as attenuated Bcl-2 expression in CAR cells. Our results support
the potentially therapeutic application of YC-1 in drug resistant oral cancer in the future.
Biography
Miau-Rong Lee has dedicated 25 years to basic Life Science Education. She was involved in the cross discipline education program sponsored by the Ministry of
Education of the Republic of China (Taiwan). Her main research interests include exploring molecular mechanisms of herbal medicine and synthetic compounds
on immunomodulation and anti-cancer effects.
Miau-Rong Lee et al., Toxicol Open Access 2017, 3:1 (Suppl)