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Page 90
Volume 08
Clinical Pharmacology & Biopharmaceutics
ISSN: 2167-065X
Pharmacology 2019
World Heart Congress 2019
August 19-20, 2019
JOINT EVENT
conferenceseries
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August 19-20, 2019 Vienna, Austria
&
7
th
World Heart Congress
24
th
World Congress on
Pharmacology
Evaluation of the effect of minocycline on resident intruder model of acute anxiety & Post Traumatic
Stress Disorder (PTSD) in golden Syrian hamsters
Shirish S Joshi
and
Panini S Patankar
GSMC & KEMH, India
Statement of the Problem
: Acute anxiety & Post Traumatic Stress Disorder are one of the most under-rated
debilitating psychiatric conditions with world-wide prevalence approximated to 41%. PTSD generally occurs after
severe psychological stress as observed in war veterans and people who have experienced severe natural or man-
made disasters resulting in features like flashbacks, insomnia, nightmares and accompanied by co-morbidities like
depression. Current line of treatment consists of short acting benzodiazepines for acute treatment along with long
term treatment with selective serotonin reuptake inhibitors (SSRI) like fluoxetine. However, current treatment has
variable efficacy & even aggregates certain symptoms like nightmares by increasing REM sleep. Thus, it is essential
to either develop a new drug or check for off label application of currently used drugs.
Methodology &Theoretical Orientation
: Resident intrusion model for induction of acute anxiety was used as this
model has greater face validity and construct validity. Hamsters were used as they are solitary and territorial animals.
All male hamsters were individually housed in ideal conditions with larger hamsters weighing >130 gm and smaller
between 80-120gm. Smaller hamsters were exposed in the cage of larger dominant ones and changes in behavior
of smaller submissive hamster were noted during five-minute pairing period. After resident intrusion, drugs were
given to the hamster intraperitoneally. Normal saline was used as disease control, lorazepam (0.5 mg/kg) as positive
control and minocycline (45 mg/kg) as test drug. The efficacy was checked by measuring serum cortisol levels within
24 hours of collection along with behavioral tests using open field and elevated plus maze (EPM).
Results & Conclusion:
Cortisol levels showed significant difference between disease control group and positive
control group. Also, there was significant difference between disease control group and minocycline group. However,
no significant difference was found in cortisol levels between positive control group and minocycline group. The
results for behavioral tests were on similar lines with time spent in open arm in EMP being significantly more in
positive control & minocycline group compared to disease control without statistical significance between positive
control group and minocycline group. Results in open field test were similar. Thus, efficacy of minocycline is similar
to lorazepam.
Clin Pharmacol Biopharm, Volume 08