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Volume 8, Issue 3 (Suppl) J Clin Cell Immunol, an open access journal
ISSN: 2155-9899 Euro Immunology 2017
June 29-July 01, 2017
June 29-July 01, 2017 Madrid, Spain
8th European
Immunology Conference
PMNs as “Trojan horse” vehicles for Brucella abortus persistence in murine bone marrow
María Cristina Gutiérrez-Jiménez, Ricardo Mora-Cartín, Carlos Chacón-Díaz, Esteban Chaves-Olarte, Edgardo Moreno and Elías Barquero-Calvo
University of Costa Rica, Costa Rica
Brucella abortus is a facultative intracellular pathogen that causes chronic infections. Neutrophils (PMNs) are the first cells that
encounter Brucella after invasion, however, Brucella resist their killing action and induce premature cell death of these leukocytes.
It has been described that B. abortus persist in bone marrow at chronic stages of infection. Nevertheless, the role of PMNs in bone
marrow persistence has not been studied. Here we show that B. abortus organisms are able to persist in murine bone marrow even at
the “declining stages of chronic infection”. B. abortus were observed inside a PMN/monocyte cell type at very low rates. Additionally,
we demonstrate that murine bone marrow PMNs phagocyte antibody-opsonized B. abortus and die quickly after infection. These
dying infected PMNs show increased adhesion and are readily taken up by RAW 264.7 macrophages. When ex vivo macrophage
infections were performed, B. abortus were more infective and replicated at higher rates when macrophages were infected through
PMNs than when infected with Brucella only. In general, proinflammatory and anti-inflammatory cytokines showed increased values
in macrophages infected through PMNs; but only after 24 hours of infection, when Brucella has already reached their replication
niche inside the cell. Our results support the notion that infected bone marrow PMN might behave as vectors for Brucella persistence
in bone marrow in a non-logistic way.
Biography
María Cristina Gutiérrez-Jiménez is a Master’s student of the University of Costa Rica. She is developing a research project on different Immunology aspects,
regarding the infectious disease called brucellosis. Her work's main objective is to study the role of polymorphonuclear neutrophils as “Trojan Horse” vehicles
during brucellosis, using a bone marrow murine model and the chronicity and persistence of the disease. She has acquired expertise in ELISA, flow cytometry,
bacterial infections, cell culture, cell differentiation, cell infection through cells and fluorescence microscopy. She has co-authored a publication regarding the role
of neutrophils during brucellosis.
María Cristina Gutiérrez-Jiménez et al., J Clin Cell Immunol 2017, 8:3(Suppl)
DOI: 10.4172/2155-9899-C1-037