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Volume 8, Issue 3 (Suppl) J Clin Cell Immunol, an open access journal
ISSN: 2155-9899 Euro Immunology 2017
June 29-July 01, 2017
June 29-July 01, 2017 Madrid, Spain
8th European
Immunology Conference
Impaired priming and increased ROS production by circulating neutrophils from patients with chronic
lymphocytic leukemia
Manukyan G1, 3, Papajik T1, Gajdos P2, Mikulkova Z1, Urbanova R1, Gabcova G1, Kudelka M2, Turcsanyi P 1, Ryznarova P1, Prochazka V1 and Kriegova E 1
1Palacky University Olomouc, Czech Republic
2Technical University of Ostrava, Czech Republic
3Institute of Molecular Biology, Armenia
Besides a classical role in antimicrobial functions, emerging evidence indicates that neutrophils could have an effect on chronic and
progressive diseases such as leukemia. Nowadays, there is limited information about the function of circulating neutrophils in the
chronic lymphocytic leukemia (CLL). The aim of the present study was to study functional properties of circulating neutrophils in
CLL. 18 CLL patients and 17 healthy controls were enrolled in the study. Priming of neutrophils with LPS as well as oxidative stress
capacity was analyzed using flow cytometry. Spontaneous and induced with fMLP and PMA oxidative stress was measured using
dihydrorhodamine 123. Despite fMLP and PMA significantly induced ROS production by neutrophils in both healthy (P<0.01) and
CLL groups (P<0.01 and P<0.001, respectively), stimulation with both inducers has led to amaximumROS-release in neutrophils from
CLL patients compared to healthy ones (P<0.05 and P<0.01, respectively). Spontaneous production of ROS by CLL neutrophils was
also increased (P<0.05) compared with healthy neutrophils. LPS up-regulated TLR2 in healthy cells (P<0.05), and TLR2 expression
was down-regulated in CLL cells (P<0.05). LPS exposure of isolated neutrophils from healthy group induced production of IL-1β and
TNF-α (P<0.05). In contrast, LPS-stimulated CLL neutrophils failed to induce releasing of both cytokines. LPS-induced production
of IL-1β and TNF-α in CLL group was lower than those released by neutrophils from healthy group (P<0.05). Taken together,
circulating neutrophils in CLL patients have altered functional properties of which may account for the heightened sensitivity to
bacterial infection as well as influence the disease course. Future studies are needed to prove our observations.
Biography
Gayane Manukyan is researcher in the National Academy of Sciences of the Republic of Armenia and a group leader of a Group of Molecular and Cellular
Immunology Institute of Molecular Biology. Currently she is postdoctoral fellow in the Department of Immunology, Medical Faculty, Palacky University, Olomouc,
Czech Republic. She has strong expertise in flow cytometry and studies focused on innate immunity and imunology of inflamatory and infectious diseases.
Manukyan G et al., J Clin Cell Immunol 2017, 8:3(Suppl)
DOI: 10.4172/2155-9899-C1-037