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Volume 8, Issue 3 (Suppl) J Clin Cell Immunol, an open access journal
ISSN: 2155-9899 Euro Immunology 2017
June 29-July 01, 2017
June 29-July 01, 2017 Madrid, Spain
8th European
Immunology Conference
Expressionprofiling of chemokine receptors inperipheral bloodmononuclear cells in chronic lymphocytic
leukemia
Gabcova G1, Kraiczova V1, Manukyan G1,2, Mikulkova Z1, Fillerova R1, Urbanova R1, Turcsanyi P1, Ryznarova P1, Papajik T 1 and Kriegova E1
1Palacky University Olomouc, Czech Republic
2Institute of Molecular Biology, Armenia
C hronic lymphocytic leukemia (CLL) cell is characterized by a progressive accumulation of long-lived CD5+ B lymphocytes
in bone marrow/lymph nodes, whose survival requires exogenous activation signals such as chemokines. Growing number of
studies show that chemokines and their receptors, in addition to trafficking, have much broader influence on neoplastic cells, such
as cell growth, differentiation and survival. To gain more insights into the chemokine receptor network in CLL, we characterized
the expression pattern of 16 canonical and 4 atypical chemokine receptors in peripheral blood mononuclear cells (PBMC) of CLL
patients (n=88) and healthy subjects (n=34) by using quantitative RT-PCR. The expression of CXCR3, CXCR4, CXCR5, CXCR7,
and CCR7 was confirmed by 6-color flow cytometry. Among deregulated receptors, 5 receptors (CCR7, CCR10, CXCR3, CXCR4,
CXCR5) were up-regulated and 9 receptors (CCR2-CCR6, CCR8, CCRL2, CXCR1, CXCR2) down-regulated in CLL; the expression
of others did not differ between CLL and controls (P>0.05). We have also analyzed differences in expression pattern in CLL groups
subdivided according to cytogenetics (13q and 17q deletions). In patients with del(17q) having worse prognosis, we observed higher
mRNA levels of CXCR6, CXCR7 and CCR10 comparing to del(13q) associated with good prognosis. In conclusion, differential
expression patterns of chemokine receptors suggest the relevance of the network of these receptors in CLL pathogenesis. The potential
of chemokine/chemokine receptor network as determinant for clinical outcome and novel therapies is worth exploring.
Biography
Gabriela Gabcova is a PhD student of Immunology at Palacky University Olomouc, Czech Republic. Her main research activity includes immunophenotyping of
cells in peripheral blood and synovial fluids by flow cytometry. She has experience with development of multi-colours panels for flow cytometry. She is primarily
focused on disorders of connective tissue (osteoarthritis, rheumatoid arthritis) and hematological malignancies (chronic lymphocytic leukemia, Hodgkin lymphoma).
Gabcova G et al., J Clin Cell Immunol 2017, 8:3(Suppl)
DOI: 10.4172/2155-9899-C1-037