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Volume 8, Issue 3 (Suppl)
J Clin Cell Immunol, an open access journal
ISSN: 2155-9899
Euro Immunology 2017
June 29-July 01, 2017
June 29-July 01, 2017 Madrid, Spain
8
th
European
Immunology Conference
Efficient production of type O foot-and-mouth disease subunit vaccine using baculovirus expression system
Chi-Chi Wen
National Pingtung University of Science and Technology, Taiwan
T
he foot and mouth disease (FMD) is a highly contagious viral disease of Artiodactyla, causing severe economy loss worldwide. In
this study, the structural proteins VP1, VP2, VP3 and VP4 of FMD serotype O were produced with the concentrations between
250 µg/mL to 550 µg/mL by using baculovirus expression vector system (BEVS). This improved the low productivity problem of
eukaryotic expression system. The concentration of the anti-FMD antibodies in animal sera can be distinguished by using purified
VP1 proteins, which were coated on the ELISA plates, showing that the recombinant VP1 protein has good antigenicity. The virus
like particles (VLPs) was observed under electric microscope by using the mixture of the four proteins and pH value adjustment.
Presently, those four structural proteins were tested alone or in mixture in pig experiment. In conclusion, this subunit vaccine has the
potential to provide protection against FMD.
Biography
Chi-Chi Wen holds a Master’s degree in Veterinary Medicine from National Taiwan University. She is a Research Fellow at Graduate Institute of Animal Vaccine
Technology of National Pingtung University of Science and Technology. Her research interest includes Molecular Biology and Biotechnology, continuing in the
research of Veterinary Medicine. She focuses on development of animal vaccines, designing new type of vaccines with biotechnology, with the hope to help animals
avoid suffering.
chi180306@gmail.comChi-Chi Wen, J Clin Cell Immunol 2017, 8:3(Suppl)
DOI: 10.4172/2155-9899-C1-037