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.com
Volume 8, Issue 3 (Suppl)
J Clin Cell Immunol, an open access journal
ISSN: 2155-9899
Euro Immunology 2017
June 29-July 01, 2017
June 29-July 01, 2017 Madrid, Spain
8
th
European
Immunology Conference
The effect of nitration on the potential allergenicity of wheat derived alpha amylase trypsin inhibitors (ATIs)
Kira Ziegler
1
, Kurt Lucas
1
, Iris Bellinghausen
2
, Fobang Liu
1
, Victor Zevallos
2
, Muhammad Ashfaq-Khan
2
, Joachim Saloga
2
, Detlef Schuppan
2
and
Ulrich Pöschl
1
1
Max Planck Institute for Chemistry, Germany
2
University of Mainz, Germany
O
ver the past decades, environmental pollution and allergy incidence have been increasing on a global scale, implicating that
they are interconnected. Air pollutants e.g. nitrogen dioxide and ozone are capable of chemically modifying airborne allergens,
particularly under humid summer smog conditions. As demonstrated for birch pollen Betv1, these nitrated allergens are known to
have enhanced allergic potential. We demonstrated that wheat derived alpha-amylase-trypsin inhibitors (ATIs), which previously
were identified as major allergens of baker’s asthma, are also strong activators of the intestinal innate immune system when ingested
with wheat products. Moreover, via their innate immune stimulatory activity, these ATIs also promote experimental allergies. As air
pollution and fertilizers can lead to nitration of these ATIs in the living grains, the aim of this project was to elucidate the effect of
nitrated ATIs on innate and adaptive immunity. Therefore, a HeLa TLR4 dual reporter cell line was stimulated with untreated ATIs
vs. nitrated ATIs. Furthermore, human monocyte-derived dendritic cells (DC) were exposed to ATIs or nitrated ATIs and changes
in specific DC maturation markers and cytokine patterns were analyzed by flow cytometry or multiplex ELISA. Additionally, T cell
proliferation after co-cultivation with different ATI-treated autologous DC was determined. In all these different
in vitro
systems
we could demonstrate a stronger stimulatory capacity of nitrated ATIs in comparison to native ATIs, indicating that nitration of an
antigen/allergen not only affects its allergenicity but also its immunogenicity.
Biography
Kira Ziegler has obtained her Diploma in Biology from the Johannes Gutenberg-University, Mainz, Germany. The topic of her thesis was “Expression and recovery
of two recombinant proteins in
Escherichia coli
for cancer vaccination”. Following this, she started her PhD at the Max Planck Institute for Chemistry, Mainz,
Germany. Her research focusses on the allergenic effect of nitration on wheat derived alpha amylase trypsin inhibitors, bridging with this topic atmospheric science
and fundamental medical research.
kira.ziegler@mpic.deKira Ziegler et al., J Clin Cell Immunol 2017, 8:3(Suppl)
DOI: 10.4172/2155-9899-C1-037