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Volume 8, Issue 3 (Suppl)
J Clin Cell Immunol, an open access journal
ISSN: 2155-9899
Euro Immunology 2017
June 29-July 01, 2017
June 29-July 01, 2017 Madrid, Spain
8
th
European
Immunology Conference
Significance of DNAmethylation to polyp formation of eosinophil and neutrophil in chronic rhinosinusitis
Jong-Yeup Kim
1
, Min-Ji Cha
1
and
Dong-Kyu Kim
2
1
Konyang University, South Korea
2
Hallym University, South Korea
P
redicting which patients are at a higher risk for recurrent chronic rhinosinusitis with nasal polyps (CRSwNP) is one of the
most challenging problems in clinical rhinology. A direct association between nasal polyp and eosinophil/neutrophil counts
was reported. This study aimed to identify difference of eosinophils and neutrophils for formation of polyp by DNA methylation in
CRS. We have previously shown that KRT 19, NR2F2, ADAMTS1, and ZNF222 levels are changed in nasal polyps (NPs) of patients
with chronic rhinosinusitis (CRS) in patients. A study was performed from 30 patients with CRS with bilateral NP, examining the
prognostic role of eosinophil and neutrophil levels. 30 patients with CRS were classified by the rate of eosinophils and neutrophils
in tissue. The methylated genes detected by DNA methylation microarray were validated by methylation-specific polymerase chain
reaction (PCR), bisulfite sequencing, and real-time PCR. DNA methylation microarray identified 43,674 CpG islands in 518 genes.
Specific genes were found to have a hypermethylated signal, and some genes were significantly hypomethylated in the promoter region
in eosinophils compared with neutrophils. Real-time PCR showed that the expression levels of genes were changed in eosinophils,
when compared with neutrophils. We clearly demonstrated that the two subgroups of CRSwNP had characteristic differences in DNA
methylation, which allows for pathophysiologically meaningful differentiations with likely therapeutic consequences. Further studies
are needed to confirm the significance of these epigenetic factors in the mechanisms underlying NP formation.
Biography
Jong-Yeup Kim has his expertise in improving the Otorhinolaryngology (snoring, septic disease, sinusitis, tonsillitis, nasal molding and allergic rhinitis). His open
and contextual evaluation model based on responsive constructivists creates new pathways for improving nose disease. He has built this model after many years
of experience in research, evaluation, teaching and administration both in hospital and education institutions.
k@kyuh.ac.krJong-Yeup Kim et al., J Clin Cell Immunol 2017, 8:3(Suppl)
DOI: 10.4172/2155-9899-C1-037