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Volume 8, Issue 3 (Suppl)
J Clin Cell Immunol, an open access journal
ISSN: 2155-9899
Euro Immunology 2017
June 29-July 01, 2017
June 29-July 01, 2017 Madrid, Spain
8
th
European
Immunology Conference
Development of genotype VII NDV inactivated vaccine
Guan-Ming Ke
National Pingtung University of Science & Technology, Taiwan
N
ewcastle disease virus (NDV), also known as avian paramyxovirus serotype 1, is a member of the family Paramyxoviridae and
causes a highly contagious respiratory, neurological, or enteric disease in chickens. Currently, genotype VII strains are the
predominant virulent strains. Although the different genotypes of NDV all belong to one serotype, it is still difficult to confer cross-
protection under stressful environmental conditions. The aim of this work is to generate an attenuated NDV strain and develop a
cell culture-derived genotype VII ND inactivate vaccine against current virulent NDV strains. After the 40
th
passage, we found that
the MDT of NDV strains is higher than 120 hours, the intracerebral pathogenicity indexes (ICPI) had decreased to 0-0.08, and
TCID50 has reached 10
8
. The attenuated strain, KGM-01, is then inactivated and mixed with oil adjuvants for vaccine evaluation.
Hemagglutination inhibition test showed that the inactivated vaccine elicited high antibody titer two weeks after immunization and
no virus shedding was detected using real-time PCR when challenged with the Sato strain and field type VII strains. Thus, KGM-01
is a low virulent, type VII genotype strain with high antigenicity suitable for inactivated NDV vaccine development
Biography
Guan-Ming Ke is a licensed veterinarian and teaches at the National Pingtung University of Science & Technology as an associate professor. He focuses on animal
vaccine development and production, continuously applying new technologies to develop new types of vaccines and to
better
the manufacturing process. His work
aims to turn research into applicable technologies that will aid in disease prevention and improve both animal and human welfare.
kegm@mail.npust.edu.twGuan-Ming Ke, J Clin Cell Immunol 2017, 8:3(Suppl)
DOI: 10.4172/2155-9899-C1-037