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.com
Volume 8, Issue 3 (Suppl)
J Clin Cell Immunol, an open access journal
ISSN: 2155-9899
Euro Immunology 2017
June 29-July 01, 2017
June 29-July 01, 2017 Madrid, Spain
8
th
European
Immunology Conference
The NOD-like receptor (NLRP3) gene variability in patients with recurrent aphthous stomatitis
Simona Valova
1
, Petra Borilova Linhartova
1
, Lucie Masopustova
1
, Jirina Bartova
2
, Jitka Petanova
2
, Pavel Kuklinek
1
, Antonin Fassmann
1
and
Lydie
Izakovicova Holla
1
1
Masaryk University, Czech Republic
2
Charles University, Czech Republic
Statement of the Problem:
Recurrent aphthous stomatitis (RAS) is a multifactorial disease with an unclear etiopathogenesis, resulting
from the interplay between genetic and environmental factors. As the dysregulation of the immune system can play a role in the RAS
development, single nucleotide polymorphisms (SNPs) in the genes for immune and inflammatory molecules were studied. The
NOD-like receptor
(NLRP3)
gene, encoding the component of the inflammasome, has been proposed as one of the candidate genes
for RAS. The aim of our study was to investigate three SNPs (rs4612666, rs10754558, rs3806265) in
NLRP3
gene in patients with RAS
and healthy controls in the Caucasian population.
Methodology:
A total of 200 Czech subjects were enrolled in this case-control study. 143 healthy controls, 57 patients with RAS
were genotyped by method based on polymerase chain reaction using 5' nuclease TaqMan® assays. Clinical parameters such as
complete blood count, levels of immunoglobulins including allergen-specific immunoglobulin E or presence of antibodies against
cytomegalovirus, Epstein-Barr virus were determined in RAS patients.
Findings:
Although no significant differences in the
NLRP3
(rs10754558, rs3806265) allele and genotype frequencies between patients
with RAS and controls were observed, statistically significant differences in
NLRP3
rs4612666 genotype frequencies between subjects
with RAS and controls were found. Carriers of
NLRP3
rs4612666 TT genotype had a higher risk of developing RAS in comparison to
subjects with CT + CC genotypes (OR=16.71, 95% CI=1.96-142.14, P=0.0024). No association between
NLRP3
haplotypes and RAS
was detected.
Conclusion & Significance:
In contrast to the previous study, associations between
NLRP3
(rs10754558, rs3806265) polymorphisms
and RAS were not confirmed. However, we suggest that
NLRP3
rs4612666 polymorphism can strongly influence the risk of developing
RAS in the Czech population.
Biography
Simona Valova studied Molecular Biology and Genetics at Faculty of Science, Masaryk University, Brno, Czech Republic. She is currently in her third year of PhD
in Physiology and Pathological Physiology at Faculty of Medicine, Masaryk University. She works in the team of Professor Lydie Izakovicova Holla that focuses on
variability in candidate genes for multifactorial diseases, including periodontitis, recurrent aphthous stomatitis, diabetes mellitus or gastroesophageal reflux disease.
simonavalova@mail.muni.czSimona Valova et al., J Clin Cell Immunol 2017, 8:3(Suppl)
DOI: 10.4172/2155-9899-C1-037