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Volume 8
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
Euro Biotechnology 2018
October 11-12, 2018
October 11-12, 2018 | Moscow, Russia
21
st
European
Biotechnology Congress
conferenceseries
.com
Page 44
Herbicide absorption and translocation in plants and soil using radioisotopes
Lydia Bondareva
and
Fedorova Nataliya
F.F. Erisman Federal Scientific Centre of Hygiene, Russia
U
nderstanding pesticide metabolism in plants and microorganisms is a key component for the development, the
safe and efficient utilization of these compounds, and for bioremediation of these chemicals in contaminated soil
and water. The use of isotopes as tracers may offer additional information, since they allow and may be differentiated
with great accuracy from the ions of the compound, which are already in the environment, even when both show a
similar chemical behavior. The liquid scintillation spectrometry technique (LSS) is knowingly the most adequate one
in order to determine the radiation with low penetration power, such as β radiation, emitted by
14
C, since it shows
adequate sensitivity and reproducibility. The use of a certified pattern for the studied herbicide is indicated for the
adequate calibration of the liquid scintillation equipment (scintillation counter). In order to quantify the radiolabeled
herbicide, the main purpose of preparing samples is obtaining a homogeneous and stable solution that is adequate for
the LSS analysis. Thus, radioisotopes was used for environmental behavior and in plants studies, since they provide
some advantages in comparison to chemical measures, including greater sensitivity, stepwise description of a particular
element in a metabolic system, and pesticide position and detection through x-ray films and/or radio image (in plants)
and liquid scintillation (in plants and soil), respectively.
Biography
Lydia Bondareva has completed her PhD from Lomonosov’s State University, Moscow, Russia. She is Senior Researcher in F.F. Erisman Federal
Scientific Centre of Hygiene, Department of the Analytical Methods of Control, Russia. She has more than 70 papers in reputed journals and has
been serving as an Editorial Board Member of repute.
lydiabondareva@gmail.comLydia Bondareva et al., J Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C5-101