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Volume 8
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
Euro Biotechnology 2018
October 11-12, 2018
October 11-12, 2018 | Moscow, Russia
21
st
European
Biotechnology Congress
conferenceseries
.com
Page 50
In vitro
studies in
Celastrus paniculatus
Willd., and green synthesis of silver nanoparticles
D H Tejavathi
and
R Niranjan
Bangalore University, India
C
elastrus paniculatus
Willd., belongs to the family Celastraceae is one of the important medicinal plants. Various
parts of the plant are used in indigenous systems of medicine for their immense health benefits. In Ayurveda, the
seeds are recognized as an effective nervine tonic. Apart from seeds, other parts like those that bark, leaf and root
are used to overcome pain and local inflammations. Because of its extensive medicinal uses, overexploitation has led
to the threatened status of the taxon.
In vitro
culture technique is one of the promising methods for conservation
and propagation of threatened taxa. Nodes, internodes and leaf were cultured on Murashige and Skoog’s medium
supplemented with various concentrations. Both direct and indirect organogenesis was obtained from the cultures. The
effective combinations of hormones for direct and indirect organogenesis were found to be MS+NAA+BA/BAP and
MS+NAA+BAP+TDZ+ - glutamine respectively. Thus, obtained shoots were rooted on MS+IBA/IAA medium and
further successfully acclimatized on soilrite. About 50% of survival rate was recorded. In view of its threatened status
and medicinal properties, synthesis of nanoparticles has a significant and promising role to play in pharmaceutics.
Hence, silver nanoparticles from seed extracts were synthesized and standard tools such as UV-Vis spectrophotometer,
SEM and XRD, characterize them. Efficacy of these nanoparticles against known pathogens is under study.
tejavathi_hanu@yahoo.comJ Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C5-101