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Volume 8
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
Euro Biotechnology 2018
October 11-12, 2018
October 11-12, 2018 | Moscow, Russia
21
st
European
Biotechnology Congress
conferenceseries
.com
Page 39
Novel
Bacillus
sp. isolates producing 2,3-butanediol have the potential to degrade lignocellulose
Kaloyan K Petrov
1
, Flora V Tsvetanova
1
, Petya V Velikova
2
and
Penka M Petrova
2
1
Institute of Microbiology - BAS, Bulgaria
2
Institute of Chemical Engineering - BAS, Bulgaria
2
,3-Butanediol (2,3-BD) is a valuable bulk-chemical with industrial applications as fuel additive and reagent in
manufacturing of moistening and softening agents, perfumes, fumigants, insecticides, explosives, plasticizers
and printing inks. The present work is dedicated to the development of bio-based process for its production by non-
pathogenic strains fromrenewable, waste, abundant, and inexpensive substrate as the lignocellulosic biomass. Ten
Bacillus
sp. strains were isolated from different soil, rhizosphere, and yogurt samples and selected for their ability to produce
2,3-butanediol from glucose. Based on 16S rRNA gene sequences, seven of them (13, 14, 16, 24, 39, 49, and 55) were
affiliated to
B. licheniformis
, two (1RA, 1RB) - to
B. cereus
group, and one strain (5RB) belonged to
B. amyloliquefaciens
group. Considering the strains potential to degrade lignocellulose, their hydrolytic enzyme activities were tested using
AZCL (azurine cross-linked) substrates. Nine strains were able to degrade cellulose, since they liquefied HE-, DEAE-
cellulose, and β-glucan. Several strains degraded the hemicellulose polysaccharides xyloglucan, xylan and arabinoxylan.
Importantly, the strains fermented the main lignocellulose monosaccharide components D-xylose, L-arabinose,
D-mannose, and D-galactose. Eight of the strains utilized branched arabinan, 7 of them - galactomannan, and 7 - inulin
(all spread in the plant biomass). Disaccharides utilization profiles revealed that all novel strains were able to ferment
sucrose, lactose, maltose, and cellobiose. In conclusion, promising non-pathogenic producers of 2,3-BD were isolated.
Displaying wide spectrum of active hydrolytic enzymes, they could be successively used in the development of a new
biotechnology for 2,3-BD production from lignocellulose, currently known as the world largest, but weakly explored
biomass source.
Biography
Kaloyan K Petrov is Head of the Department of Chemical and Biochemical Reactors in the Institute of Chemical Engineering, Bulgarian Academy
of Sciences. His work is devoted to optimization of downstream processes by the techniques of bioprocess and metabolic engineering. The team
develops biotechnologies for microbial production of platform chemicals and fuels by conversion of waste or renewable energy resources, including
the cutting-edge biotechnologies for 2,3-butanediol production from glycerol and starch by the use of natural and recombinant strains, and novel
bio-processes for lactic acid synthesis by utilization of starch and inulin. Other topics of his work are the fermentative production of enzymes and
valuable chemicals in respect of their industrial application, strain’s improvement by gene engineering, microbiological and molecular biological tools,
development and analysis of probiotics and prebiotics.
kaloian04@yahoo.comKaloyan K Petrov et al., J Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C5-101