22 / 22
Volume 8
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
Euro Biotechnology 2018
October 11-12, 2018
October 11-12, 2018 | Moscow, Russia
21
st
European
Biotechnology Congress
conferenceseries
.com
Page 58
Evaluation of diagnostic potential of recombinant D-erythrulose 1-phosphate dehydrogenase
using indirect enzyme-linked immunosorbent assay for diagnosis of bovine brucellosis
W S A A Shell
1
, M S Diab
1
, A A Samy
2
and
J Eljakee
3
1
Central Laboratory For Evaluation Of Veterinary Biologics, Egypt
2
National Research Centre Dokki, Egypt
3
Cairo University, Egypt
S
erological tests used for diagnosis of bovine brucellosis are usually depending on smooth lipopolysaccharides (S-LPS)
as a diagnostic antigen which usually gives false positive reactions. So, our study aims to produce and evaluate a
diagnostic kit for accurate diagnosis of bovine brucellosis differentiating between vaccinated and infected cattle and
exclusion of false positive cases. Idea of this kit depends upon the fact that The
EryC
gene is absent in
Brucella abortus
S19 only but it is present and functional in all other
Brucella
strains and isolates so according to these facts, the use of
ELISA kit coated with single subunit (recombinant)
EryC
protein may be useful, rather than S-LPS, as an alternative
diagnostic antigen in diagnosis of bovine brucellosis and differentiation between S19 vaccinated and
Brucella
infected
cattle. The present study evaluated antibody responses of brucellosis infected and S19 vaccinated cattle to purified
recombinant
EryC
protein in an indirect enzyme-linked immunosorbent assay (I-ELISA). Cattle sera were screened
using Rose Bengal Plate test (RBPT). 114 samples of naturally infected cattle (Rose Bengal test positive), 78 sera from
S19 vaccinated cattle and 25 sera samples from
Brucella
free cattle were used in this study. I-ELISA using S-LPS and
periplasmic proteins as a coating antigen were used as a gold standard test. The results revealed that in case of sera of
naturally infected cattle, sero-positivity was 94.7%, 100%, 100% and 100% with
EryC
-ELISA, LPS-ELISA, periplasmic-
ELISA and Rose Bengal test respectively. Where in case of sera of S19 vaccinated cattle, all samples were negative when
tested with
EryC
-ELISA while in case of LPS-ELISA, periplasmic-ELISA and rose Bengal test, sero-positivity was 92.3%,
84.6% and 100% respectively. It could concluded that the
EryC
protein could be used in serological tests for diagnosis of
bovine brucellosis and differentiation between infected and
Brucella abortus
S19-vaccinated cattle but more studies are
needed to be done on large cattle populations accompanied with bacteriological isolation to detect the sensitivity and
specificity of this protein as a diagnostic antigen and also for validate this test.
tarikwaleedshell@hotmail.comJ Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C5-101