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Volume 8
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X Euro Biotechnology 2018
October 11-12, 2018
October 11-12, 2018 | Moscow, Russia
21st European
Biotechnology Congress
conferenceseries.com
Page 43
Thermostable catalase as an excellent supplement for growth media of industrially important
lactic acid bacteria strains
Eva Struharnanska1, Monika Chovanova1, Zdenko Levarski1,2, Marcel Zamocky3, Stanislav Stuchlik1,2 and Jan Turna1,2
1Comenius University in Bratislava, Slovakia
2Comenius University Science Park, Slovakia
3Slovak Academy of Science, Slovakia
Lactic acid bcteria (LAB) are exceptionally important strains in food industry. It is a heterogeneous group of Gram-
positive bacteria that are acid-resistant, generally non-sporing, microaerophilic bacilli or cocci which have the same
metabolic andphysiological properties including the ability todegrade carbohydrates andproduce lactic acid. LAB include
many genera, e. g. Oenococcus, Sporolactobacillus, Teragenococcus, Vagococcus, Lactobacillus, Leuconostoc, Pediococcus,
Lactococcus, Streptococcus, Aerococcus, Carnobacterium, Enterococcus and Weisella. They are usually catalase-negative
strains, which represents a big disadvantage in food production in comparison with pathogenic bacteria as staphylococci
and listeria existing in the same environment, because one of the most used disinfection agent is hydrogen peroxide that
is utilized by catalases. We focused on increase in LAB surviving through the disinfection using thermostable catalase
(produced in our laboratory) in growth media. In our functional test 10 mM hydrogen peroxide was applied to bacteria
and this suspension was cultivated for 60 minutes. 10 mM thermostable catalase AfKatG was added to solid media to
cultivate bacteria afterwards. Our control samples were cultivated without catalase addition. As predicted there was
no difference in the growth of pathogenic bacteria with or without catalase addition to media. However, we showed a
huge positive effect on surviving LAB. With addition of catalase to solid media we gained 2-38 times higher number
of surviving colonies than in control samples without catalase. We can assume catalase as an excellent supplement for
growth media of food strains.
Biography
Eva Struharnanska has completed her Master degree in Biotechnology at the Comenius University in Bratislava, Faculty of Natural Sciences,
Department of Molecular Biology in 2015. She has also completed her Master degree in Pharmacy at the Comenius University in Bratislava, Faculty
of Pharmacy in 2017. She started her PhD studies in Biotechnology after graduation in 2015 at the same Department of Molecular Biology. The theme
of her studies: New strategies in production of recombinant proteins; and she focuses on production and purification of a thermostable catalase.
Eva Struharnanska et al., J Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C5-101