euro-biotechnology-2018-posters-abstracts

6 / 22

Notes:
Volume 8
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X Euro Biotechnology 2018
October 11-12, 2018
October 11-12, 2018 | Moscow, Russia
21st European
Biotechnology Congress
conferenceseries.com
Page 42
Luciferase of the syllid polychaete Odontosyllis undecimdonta
Ekaterina S Shakhova1, Alexey A Kotlobay1, Darrin T Schultz2, Ilia V Yampolsky1,3 and Yuichi Oba4
1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russia
2University of California Santa Cruz, USA
3Pirogov Russian National Research Medical University, Russia
4Chubu University, Japan
B ioluminescence is one of the most beautiful and fascinating natural phenomena. Methods for biological and medical
research and various techniques based on luciferin-luciferase reactions play an important role in modern science
and are widely used from analytical methods in vitro and in vivo, including tests for various analytes, to real time bio-
imaging of live systems. Odontosyllis undecimdonta is a marine syllid polychaete that produces bright internal and
exuded bioluminescence. Despite over fifty years of biochemical investigation into Odontosyllis bioluminescence, the
light-emitting small molecule substrate and catalyzing luciferase protein have remained a mystery. Here we describe
the O. undecimdonta luciferase, its amino acid sequence and some biochemical properties. Moreover, no homologous
proteins in publicly available datasets were identified. This suggests that the syllid polychaetes possess an evolutionarily
unique luciferase among all characterized luminous taxa.
Biography
Ekaterina S Shakhova is student of biological faculty at Moscow State University
Ekaterina S Shakhova et al., J Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C5-101