20 / 22
Volume 8
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
Euro Biotechnology 2018
October 11-12, 2018
October 11-12, 2018 | Moscow, Russia
21
st
European
Biotechnology Congress
conferenceseries
.com
Page 56
Enhancement of morphogenetic potential to obtain elite varieties of
Sauropus androgynus
(L.)
Merr. through somatic embryogenesis
S Padma
1
and
D H Tejavathi
2
1
MES College, Bangalore, India
2
Bangalore University, India
S
. androgynus
is a member of Euphorbiaceae, popularized as multivitamin plant and consumed as green leafy vegetable
due to its rich nutritional profile including proteins, vitamins, minerals, essential amino acids, etc. The plant is
cautioned for excessive consumption due to the presence of papaverine alkaloid which, at higher concentration leads to
bronchiolitis obliterans. In the present study morphogenetic potential of shoot tip, leaf and nodal explants of
Sauropus
androgynus
was investigated to develop and enhance the reliable plant regeneration protocol via somatic embryogenesis.
Somatic embryos were derived directly and from the embryogenic callus derived from shoot tip, node and leaf cultures
on Phillips and Collins (L
2
) medium supplemented with NAA at various concentrations ranging from 5.3 µM/l-26.85
µM/l within two months of inoculation. Thus obtained embryos were sub cultured to modified L
2
media supplemented
with increased vitamin level for further growth. Somatic embryos with well-developed cotyledons were transferred to
normal andmodified L2 basal medium for conversion.The plantlets thus obtained were subjected to brief acclimatization
before transferring them to land. About 95% of survival was recorded. Optimized techniques of various explant
cultures on Phillips and Collins media with various growth regulators has supplemented the conventional propagation
methods in commercial production resulting in availability of improved
Sauropus
through somatic embryogenesis. The
development of regeneration systems for
S. androgynous
has opened possibilities for developing genotypes with novel
characters including low quantity papaverine content which has facilitated conventional improvement programs there
by providing a valuable resource to the food and pharmaceutical industry. Based on this research, plant tissue culture
techniques show promise for economical and convenient application in
Sauropus androgynus
breeding.
padmaseenappa@gmail.comJ Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C5-101