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Volume 8
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
Euro Biotechnology 2018
October 11-12, 2018
October 11-12, 2018 | Moscow, Russia
21
st
European
Biotechnology Congress
conferenceseries
.com
Page 57
Formulation and anticancer evaluation of beta-sitosterol in henna methanolic extract embedded
in controlled release nanocomposite
Sanjukta Badhai
1
, Durga Barik
2
and
Bairagi C Mallick
2
1
KIIT University, Bhubaneswar, India
2
Ravenshaw University, India
I
n the present study, beta-sitosterol in Lawsonia methanolic leaf extract embedded in controlled release nanocomposite
was prepared and evaluated for in vivo anticancer efficacy in dimethyl hydrazine (DMH) induced colon cancer. In
the present study, colon cancer was induced by subcutaneous injection of DMH (20 mg/kg
b.wt) for 15 weeks. The
animals were divided into five groups as follows control, DMH alone, DMH and beta sitosterol nanocomposite (50
mg/kg), DMH and Beta Sitosterol nanocomposite (100 mg/kg) and DMH and standard silymarin (100 mg/kg) and
the treatment was carried out for 15 weeks . At the end of the study period the blood was withdrawn and serum was
separated for haematological, biochemical analysis and tumor markers. Further, the colonic tissue was removed for the
estimation of antioxidants and histopathological analysis. The results of the study displays that DMH intoxication elicits
altered haematological parameters (RBC,WBC and Hb), elevated lipid peroxidation and decreased antioxidants level
(SOD, CAT, GPX, GST and GSH), elevated lipid profiles (cholesterol and triglycerides), tumor markers (CEA and AFP)
and altered colonic tissue histology. Meanwhile, treatment with beta sitosterol nanocomposites significantly restored
the altered biochemicals parameters in DMH induced colon cancer mediated by its anticancer efficacy. Further, beta
sitosterol nanocomposite (100 mg/kg) showed marked efficacy.
sanjukta.badhai@gmail.comJ Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C5-101