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Euro Biotechnology 2016
November 07-09, 2016
Volume 6, Issue 7(Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
conferenceseries .com
November 07-09, 2016 Alicante, Spain
12th Euro Biotechnology Congress
R Sowmya et al., J Biotechnol Biomater 2016, 6:7(Suppl)
Influence of AM fungi and Trichoderma viride on growth and active principles in micropropagated
Bacopa monnieri (L.) Pennell, a medicinal plant
R Sowmya1 and D H Tejavathi2
1Mysore University, India
2Bangalore University, India
Bacopa monnieri (L.) Pennell is commonly known as “Brahmi” belongs to the family Scrophulariaceae. It is one of the most
important medicinal plants, which has fascinated the fields of traditional and modern system of medicine as memory
enhancer and nervine tonic. To understand the influence of AM fungi alone and with a phosphate solubilizing organism on
micropropagated plant in comparison with normal plants, an attempt has been made in the present study. Micropropagated
plants were raised onMSmediumsupplementedwithKin and BAP from leaf explants. Both normal andmicropropagated plants
were inoculated with Glomus mosseae and G. fasciculatum alone and in combination with Trichoderma viride in pots. Percent
colonization and spore count were recorded in both normal and micropropagated treated and control plants. Micropropagated
plants showed early and better colonization with AMF association compared to normal plants. Plant growth and biomass
were recorded for treated and control of normal and micropropagated plants. AMF enhanced the growth of micropropagated
plants considerably with G. mosseae in combination with T. viride compared to control plants. Micropropagated inoculated
plants showed increased level of chlorophylls, total proteins and total phenolics but reducing sugars and total carbohydrates
content showed decreased level compared to control plants. The increased level in the Bacoside A-content was recorded in
plants treated with G. mosseae along with T. viride . The micropropagated B. monnieri plants responded excellently to the AMF
inoculation with T. viride and showed better establishment in the pots by availing good growth and improved physiological
condition.
Biography
R Sowmya is currently an Assistant Professor, Department of Botany of Yuvaraja’s College, University of Mysore, India. She has obtained her MSc from
the Bangalore University in the year 1994 and MPhil in 1996. She has completed her PhD with the thesis entitled “Utilization of VAM fungi for improving the
establishment of micropropagated plants” in 2002 under the guidance of Prof. D. H. Tejavathi. She continued her research as a Research Associate in the CSIR
Project “Commercial exploitation of regenerates of Agave”. She has published research papers in national and international journals and presented her research
work in national and international conferences.