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Euro Biotechnology 2016
November 07-09, 2016
Volume 6, Issue 7(Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
conferenceseries .com
November 07-09, 2016 Alicante, Spain
12th Euro Biotechnology Congress
Giorgi Kvesitadze et al., J Biotechnol Biomater 2016, 6:7(Suppl)
Stable cellulose degrading enzymes from thermotolerant fungi strains
Giorgi Kvesitadze1, L Kutateladze1, R Khvedelidze1, T Urushadze1, N G Zakariashvili1, N Tsiklauri1, I Khokhashvili1, M Jobava 1, T Aleksidze1, T Burduli1, T
Sadunishvili1 and S Byoung-In2
1Agricultural University of Georgia, Georgia
2Hanyang University, South Korea
Search for energy from renewable resources is more urgent now than ever. In natural environments, fungi are the primary
degraders of lignocellulosic biomass, excreting both hydrolytic and oxidative enzymes. The majority of cellulases used in
biotechnology are still derived fromwell-characterized non-extremophilic microorganisms and there is a very little information
regarding cellulases from extremophiles. An important drawback of these commonly used industrial enzymes is the lack
of activity at even slightly elevated temperature and the tendency of these enzymes to denature at elevated temperatures or
other critical conditions. Project is focused on obtaining stable enzymes from Durmishidze Institute of Biochemistry and
Biotechnology, AUG unique extremophilic mycelial fungi collection for the creation of biotechnology of production of
fuel-bioethanol from agricultural and industrial lignocellulosic wastes. Cellulase/xylanase producers, mesophil Penicillium
canescence D 85 and thermophil Sporotrichum pulverulentum T 5-0 synthesizing extracellular enzymes with activities 185
U/g/1600 U/g and 110 U/g/840 U/g, correspondingly, have been selected. Optimum pH of action of the studied cellulase/
xylanases was similar and equaled to 4.5-5.0. Simultaneously 21 basidial fungi strains have been selected as laccase producers.
Using these enzymes allowed creation of the technology of glucose production from agricultural wastes by hydrolyzing of
cellulose up to 80% or higher.
Biography
Giorgi Kvesitadze has completed his PhD from Bach Institute of Biochemistry, Moscow and Postdoctoral research at University of Pennsylvania, Philadelphia and
Lehigh University, Bethlehem, USA. He is the Director of the Durmishidze Institute of Biochemistry and Biotechnology and Professor at AUG. He has been elected
as a Member of GNAS in 1988. He is the author of more than 110 papers in reputed journals and has been serving as an Editorial Board Member of International
journals: Ecotoxicology and Environmental Safety, Fresenius Environmental Bulletin, EuroBiotech Journal, Journal of Biological Physics and Chemistry, Annals of
Agrarian Sciences and of national journal: Bulletin of the Georgian NAS .