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Page 62

Euro Biotechnology 2016

November 07-09, 2016

Volume 6, Issue 7(Suppl)

J Biotechnol Biomater

ISSN: 2155-952X JBTBM, an open access journal

conferenceseries

.com

November 07-09, 2016 Alicante, Spain

12

th

Euro Biotechnology Congress

Tinatin Sadunishvili et al., J Biotechnol Biomater 2016, 6:7(Suppl)

http://dx.doi.org/10.4172/2155-952X.C1.065

Isolation of

Cms

specific bacteriophages

Tinatin Sadunishvili

1

, D Gaganidze

1

, T Burbutashvili

1

, N Sturua

1

, N Amashukeli

1

, Sh Kharadze

1

, M Gamkrelidze

1

, L Amiranashvili

1

and A Maciejewska

2

1

Agricultural University of Georgia, Georgia

2

Plant Breeding and Acclimatization Institute, Poland

B

acterial ring rot is a highly infectious disease of potato caused by

Clavibacter michiganensis

subsp.

sepedonicus

(

Cms

). Crop

losses caused with ring rot range from 11 to 44% in different countries and significantly increases during potato storage. In

EUmember states

Cms

is under strict statutory control. Management of ring rot of potato is especially difficult in storage places,

where the pathogen, being in a latent form, may infect almost all tubers. Some disinfectants or fumigants are recommended

on seed potatoes and during storage. Achieving sustainable agriculture necessitate the search for safer, more specific and

environment-friendly control methods. The aim of the project is to identify the spread of potato ring rot in Georgia, isolate the

pathogen and its specific bacteriophages for their biological control. Field studies in 5 potato production regions did not reveal

ring rot disease; though there were some tubers in potato storage houses carrying

Cms

, which was confirmed by molecular

detection method. Several pure

Cms

isolates were recovered from these samples and confirmed by specific PCR they are

Cms

;

their cells shapes, colony morphology and biochemical tests have been studied. Collected soil and diseased potato samples

were checked on

Cms

Georgian and Polish isolates on phage content. Four bacteriophages were obtained. Phagosensitivity of

the isolates to phages have been studied. Phages #8 and 13 lyse almost all Georgian and Polish

Cms

isolates.

Biography

Tinatin Sadunishvili has completed her PhD from Durmishidze Institute of Biochemistry and Biotechnology, Agricultural University of Georgia (AUG) and Postdoctoral

research at Institute of Enzymology and Etvos Lorand University, Budapest. She is the Professor and Head of Laboratory of Plant Biochemistry and Biotechnology

at Durmishidze Institute of Biochemistry and Biotechnology, AUG. She has been elected as a Member of GNAS in 2015. She has published more than 60 papers

in reputed journals and has been serving as an Editorial Board Member of journals:

Bulletin of the Georgian NAS, Annals of Agrarian Sciences

and

Microbiology

and Biotechnology

(Tbilisi).

t.sadunishvili@agruni.edu.ge