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Euro Biotechnology 2016
November 07-09, 2016
Volume 6, Issue 7(Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
conferenceseries .com
November 07-09, 2016 Alicante, Spain
12th Euro Biotechnology Congress
Iveta Megrelishvili et al., J Biotechnol Biomater 2016, 6:7(Suppl)
Investigation of potato viral diseases in Georgian region Akhalkalaki
Iveta Megrelishvili, Maia Kukhaleishvili, Ekaterine Bulauri and Tamar Chipashvili
Georgian Technical University, Georgia
To avoid virus infection of seed potatoes apical meristemmethod is used all over the world which allows receiving virus free
plants. The main problem in seed potato industry is viral contamination. Potato virus symptoms are not always revealed
immediately, it depends on such factors as growth conditions, season, time of plant infection, existence of virus vectors, etc.
There are number of viruses which affect the plant productivity, they can cause significant decrease in yield. In spite of diverse
climatic conditions, potato is high spreading culture in mountain regions of Georgia. The main goal of the research was to
study the potato viral diseases distribution in Georgia. Survey for the detection of viral agents was conducted from potato
varieties; Agria, Impla, Nevsky, Marfona located in Alkalaki region. Potato cultivars were tested for 6 types of viruses: PVA,
PVS, PLRV, PVY, PVM, PVX by means of Double Antibody Sandwich-Enzyme Linked Immunosorbent Assay (DAS-ELISA)
using monoclonal and polyclonal antibody. The result showed that PVY and PLRV virus presence respectively: 27.8% and
18.6% in collected samples was relatively high comparing to others. PVM virus distribution was minority (2.45%) and existence
of PVX, PVA and PVS was not revealed. In the same samples were found double infection particularly 8.3% of patterns were
containing PVY/PLRV combination. Therefore, based on the result of our study it can be concluded that the only way to avoid
reduced harvest yields is regular potato seed production sampling and testing of all stage (lab, greenhouse & open field).
Biography
Iveta Megrelishvili has completed his PhD from Ivane Javakhishvili Tbilisi State University, Georgia. She is the main research scientist of Georgian Technical
University, Biotechnology Center and Head of Virology Lab, Scientific-Research Center of Agriculture. He has published more than 12 papers in reputed journals
and has great experiences in plant biotechnology sphere.