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Volume 6, Issue 4 (Suppl)
Clin Pharmacol Biopharm, an open access journal
ISSN: 2167-065X
Page 94
Euro Biopharma & Ethnopharmacology 2017
November 09-11, 2017
&
6
th
International Conference and Exhibition on
November 09-11, 2017 Vienna, Austria
4
th
EUROPEAN BIOPHARMA CONGRESS
PHARMACOLOGY AND ETHNOPHARMACOLOGY
Joint Event
Sarcopoterium spinosum
: An antidiabetic medicinal plant with a novel mechanism of action
T Rosenzweig, K Rozenberg
and
N Skalka
Ariel University, Israel
S
arcopoterium spinosum
(
S. spinosum
) is an abundant plant in Israel, used by Bedouin traditional medicine for the treatment
of diabetes. In our previous studies the glucose lowering properties of this herb were validated
in vitro
and in-vivo. The
goal of this study is to clarify the mechanisms of action mediating the effects of S.spinosum on glucose uptake.
S. spinosum
facilitates glucose uptake by a unique mechanism, different from that induced by either insulin or metformin;
S. spinosum
increased glucose uptake by 3T3-L1 adipocytes in a mechanism involving Glut4 translocation, independent of AMPK or PI3K
activity. Akt activation is required to induce
S. spinosum
-dependent glucose uptake, however its mechanism of activation is
still unclear; while neither ser473 nor thr308 were phosphorylated by
S. spinosum
, translocation of Akt from cytoplasm to
membrane and nucleus was detected. In addition, substrates of Akt were phosphorylated by the extract. The hypothesis that
S.
spinosum
utilizes a different set of proteins to induce glucose uptake was supported by results demonstrating that differentiating
adipocytes respond differently to insulin and
S. spinosum
; while insulin gradually enhanced glucose uptake from the 11th day
of differentiation,
S. spinosum
increased glucose uptake from the 8th day of differentiation. In addition,
S. spinosum
and insulin
had additive effect on glucose uptake in fully differentiated adipocytes. Phosphoproteomics of serine/threonine residues
phosphorylated by
S. spinosum
followed by bioinformatic analysis indicate for the activation of insulin-receptor pathway. We
conclude that active ingredients in
S. spinosum
activate insulin signaling by a unique mechanism. Clarifying this mechanism
of action may lead to the development of new agents for the treatment of diabetes.
tovitro@ariel.ac.ilClin Pharmacol Biopharm 2017, 6:4(Suppl)
DOI: 10.4172/2167-065X-C1-026