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Analytica 2016
September 28-30, 2016
Volume 7, Issue 5(Suppl) J Anal Bioanal Tech 2016
ISSN: 2155-9872 JABT, an open access journal
conferenceseries .com
September 28-30, 2016 Orlando, USA
7th International Conference and Exhibition on
Analytical & Bioanalytical Techniques
Ling Huang, J Anal Bioanal Tech 2016, 7:5(Suppl)
Assessment of Feng-Liao-Chang-Wei-Kang as a potential inducer of cytochrome P450 3A4 and
pregnane X receptors
Ling Huang
Hainan Medical University, China
Feng-Liao-Chang-Wei-Kang (FLCWK) is a traditional Chinese patent medicine that mainly consists of Daphniphyllum
calycinum roots and Polygonum hydropiper . As a complex containing several types of flavonoids, FLCWK has potential
effects on the drug metabolism enzyme P450 3A4(CYP3A4) and nuclear receptors. This study aims to explore the effects of
FLCWK on CYP3A1 (CYP3A4's homolog in rats) in rats and to determine whether FLCWK could participate in the processes
of hPXR- and hCAR-mediated transactivation of CYP3A4. The effects of FLCWK on CYP3A1 mRNA, protein expression and
catalytic activity levels in Sprague-Dawley(SD) rat liver tissues were detected using real-time PCR, western blotting and high-
performance liquid chromatography (HPLC) assays. The effects of hPXR and hCAR on CYP3A4 transcriptional activity were
examined using luciferase reporter gene assays. Futher study of FLCWK on the CYP3A4 gene expression mediated by PXR
pathway was investigated by transient transfection of PXR siRNA. This study found that FLCWK could significantly increase
the CYP3A1 mRNA gene and protein expression levels and CYP3A1 activity in SD rats. In PXR-CYP3A4 co-transfected
cells, FLCWK could significantly induce CYP3A4 luciferase activity mediated by PXR. PXR-knockdown (transfected with
siPXR construct) decreased the CYP3A4 mRNA level than in the control cells transfected with corresponding vector. Taken
together, these findings suggest that FLCWK could significantly up-regulate CYP3A4 levels via the PXR-mediated pathway.
This effect should be taken into consideration to predict any potential drug-drug interactions between FLCWK and other co-
administered drugs.
Biography
Ling Huang has completed her PhD from School of Pharmacy of Sun Yat-sen University. She has published more than 10 papers in reputed journals and has been
serving as an Editorial Board Member of repute.