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Analytica 2016
September 28-30, 2016
Volume 7, Issue 5(Suppl) J Anal Bioanal Tech 2016
ISSN: 2155-9872 JABT, an open access journal
conferenceseries .com
September 28-30, 2016 Orlando, USA
7th International Conference and Exhibition on
Analytical & Bioanalytical Techniques
Yi Ding et al., J Anal Bioanal Tech 2016, 7:5(Suppl)
Method development of carbohydrate profile for Abbott nutrional products using HPAEC/PAD
Yi Ding and Chunyan Zhang
Abbott Nutrition Research and Development, Singapore
The purpose of this research method is to determine sugar profile (mono- and disaccharides) of Abbott nutritional products
in fulfillment of the “Sugar” label claim. In thismethod, the sugars (galactose, glucose, fructose, sucrose, lactose andmaltose)
were extracted from product primarily by dilution in water. The sugars were analyzed via high performance anion exchange
coupled with pulsed amperometric detection (HPAEC/PAD). The HPAEC/PAD Dionex ICS5000 system was equipped with a
triple pulsed electrochemical cell (ED) with a pH reference electrode (Ag/AgCl), a gold working electrode, and a borate trap in
tandem with a PA1 analytical column. Quantitation was accomplished using a six level quadratic curve and peak area. Sugars
were analyzed by HPAEC/PAD via a new gradient elution program to ensure adequate resolution of the analytes of interest and
known interferences from AN commodities. Method specificity was successfully evaluated by comparing the retention times
of a sugar standard mix vs. commonly used commodities and ingredients. Method accuracy and precision were evaluated by
comparing results generated for AN research samples using this research method relative to third party laboratory results.
Biography
Yi Ding has completed his PhD in Chemistry from Division of Chemistry and Biological Chemistry, Nanyang Technological University, Singapore in 2010. He is
currently a Senior Analytical Scientist of Abbott Nutrition Research and Development, Singapore.